Department of Biochemistry

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Browsing Department of Biochemistry by Subject "Aeration"

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    Decolorization Kinetics of Reactive Blue 13 Azo Dye by Proteus mirabilis
    (CSIR-NEERI, 2014-10) Olukanni, Olumide
    A dye decolorizing bacterium, Proteus mirabilis, isolated from soil was used in batch experiments for decolorization of the sulphonated dye, Reactive blue 13. Effects of initial substrate (dye) and bacterial cell mass concentrations on the specific decolorization rate and extent of dye decolorization were investigated. Aeration as against agitation increased the decolorization rate by 4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye concentration 382.98 mg/L. The decolorization rate by Proteus mirabilis was found to follow half (0.5498) and first (1.0977) order kinetics with respect to dye concentration and cell mass concentrations respectively. The specific decolorization rate estimated from the experimental data was 37.03 mg/L/h/g dry cell mass (DCM), and the value of the apparent Km was 284.76 mg/L. This study showed that the strain of Proteus mirabilis has potential in wastewater color removal.
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    Decolorizing Kinetics of Reactive Blue 13 Azo Dye by Proteus mirabilis
    (NEERI J Environ Sci Engg, 2014-10) Olukanni, Olumide
    A dye decolorizing bacterium, Proteus mirabilis, isolated from soil was used in batch experiments for decolorization of the sulphonated dye, Reactive blue 13. Effects of initial substrate (dye) and bacterial cell mass concentrations on the specific decolorization rate and extent of dye decolorization were investigated. Aeration as against agitation increased the decolorization rate by 4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye concentration 382.98 mg/L. The decolorization rate by Proteus mirabilis was found to follow half (0.5498) and first (1.0977) order kinetics with respect to dye concentration and cell mass concentrations respectively. The specific decolorization rate estimated from the experimental data was 37.03 mg/L/h/g dry cell mass (DCM), and the value of the apparent Km was 284.76 mg/L. This study showed that the strain of Proteus mirabilis has potential in wastewater color removal.