Decolorizing Kinetics of Reactive Blue 13 Azo Dye by Proteus mirabilis
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Date
2014-10
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Publisher
NEERI J Environ Sci Engg
Abstract
A dye decolorizing bacterium, Proteus mirabilis, isolated from soil was used in
batch experiments for decolorization of the sulphonated dye, Reactive blue 13. Effects of
initial substrate (dye) and bacterial cell mass concentrations on the specific decolorization
rate and extent of dye decolorization were investigated. Aeration as against agitation
increased the decolorization rate by 4-fold. The dependence of the decolorization rate on
initial dye concentration essentially followed Monod-type kinetics and the maximal rate
occurred with the dye concentration 382.98 mg/L. The decolorization rate by Proteus
mirabilis was found to follow half (0.5498) and first (1.0977) order kinetics with respect
to dye concentration and cell mass concentrations respectively. The specific
decolorization rate estimated from the experimental data was 37.03 mg/L/h/g dry cell
mass (DCM), and the value of the apparent Km was 284.76 mg/L. This study showed that
the strain of Proteus mirabilis has potential in wastewater color removal.
Description
Investigation on initial substrate (dye) and bacterial cell mass concentrations on the specific decolorization
rate.
Keywords
Kinetics, Decolorization, Proteus mirabilis, Aeration, Azo dyes, Reactive Blue 13, Biodegradation