Department of Biochemistry

Permanent URI for this collection

https://repository.run.edu.ng/handle/123456789/77

Browse

Browsing Department of Biochemistry by Author "Salau, Bamidele"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Chemopreventive and Remediation Effect of Hydrocotyl bonariensis Comm. Ex Lam (Apiaceae) Leave Extract in Galactose-induced Cataract
    (Elsevier, 2009-05-04) Salau, Bamidele
    Ethnopharmacological relevance: Hydrocotyl bonariensis Comm. Ex Lam (Apiaceae) is being widely used in Western Nigeria in treating various symptoms of ophthalmic diseases; however scientific data in support of this medicinal use have not been reported. Aim of the study: This study, investigated the efficacy of Hydrocotyl bonariensis leave extract in offering protection against experimental cataract and also examined its remediation effect when administered after cataract onset. Materials and methods: Weanling albino rats fed with 30% galactose diet were used in the study. Mechanisms of action of the extract were investigated by measuring the degree of lens peroxidation, lens antioxidant status and lens protein concentration. Severity of cataract was determined by measuring the cataract index. Results: The extract at 500 mg kg−1 reduced cataract index significantly and also reduced cataract progression when administered after cataract onset. Administration of this dosage also significantly reduced the degree of lens peroxidation, increased the level of reduced glutathione (GSH) and the lens catalase and superoxide dismutase activity. The extract also prevents protein insolubilization. Administration of the extract at 1000 mg kg−1 reduced cataract index and lens peroxidation but did not increase the antioxidant status significantly. Administration of the extract after cataract onset reduced cataract index, moderately increased percentage soluble protein above the value prior to the arrest of hypergalactosemia but did not increase the antioxidant status. Conclusion: Our study suggests that Hydrocotyl bonariensis protects against galactose-induced cataract, and that administration of the extract after cataract onset reduced cataract progression but did not reverse cataractogenesis.
  • Thumbnail Image
    Item
    Fruits and Vegetables Moderate Lipid Cardiovascular Risk Factor in Hypertensive Patients
    (BioMed Central, 2006-12) Salau, Bamidele
    Hyperlipidemia is a major risk factor in etiology of cardiovascular disease. Previous studies have shown association between vegetarian diet and low total serum cholesterol as well as LDL-cholesterol which is a pointer to low risk of cardiovascular disease. Dietary fiber, antioxidants and other classes of nutrients have been reported to ameliorate cardiovascular risk factors. Fruits and vegetables being rich sources of fiber and antioxidants have been the focus in intervention studies. The current work reports the effect local fruits and vegetables on cardiovascular risk factors in African hypertensive subjects in an 8 week study. Though there was no significant difference in the Body Mass Index and HDL-cholesterol at the end of the eighth week, there were significant reductions (P < 0.05) in serum triglycerides (125.87 ± 6.0 to108.27 ± 5.49 mgdL-1); total serum cholesterol (226.60 ± 6.15 to 179.20 ± 5.78) and LDL-cholesterol (135.69 ± 5.56 to 93.07 ± 7.18 mgdL-1). We concluded that consumption of combination of local fruits and vegetables may reduce the incidence of cardiovascular risk factors in Africans.
  • Thumbnail Image
    Item
    Methanolic Extract of Musa Sapientum Sucker Moderates Fasting Blood Glucose and Body Weight of Alloxan Induced Diabetic Rats.
    (Society of Applied Sciences, 2010) Salau, Bamidele
    Effect of methanolic extract of Musa sapientum Linn. Sucker on fasting blood glucose, body weight and pancreas histology of alloxan induced hyperglyceamic rats was evaluated and compared with that of reference drug, glibenclamide. The fasting blood glucose was measured using one touch life scan glucometer; body weight was monitored with digital scale balance, while histology of pancreas was investigated after staining with heamatoxylin-eosin dye. The extract at all tested doses (5 mg and 10 mg kg-1/day) significantly (p<0.05) lowered fasting blood glucose level in the treated rats compared with the diabetic but untreated rats (test control). At 10 mg kg-1 administration of the extract, a significant increase in body weight was observed compared with the diabetic untreated group. The pancreas histology indicates significant recovery with the extract administration. The efficacy of the extract in reducing blood glucose level, improving body weight and rejuvenating the damaged pancreas of alloxan induced diabetic rats favourably compared with that of reference drug, glibenclamide.