Characterization of Plasmodium falciparum Structure in Nigeria with Malaria SNPS Barcode.

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dc.contributor.authorFolarin, Onikepe
dc.date.accessioned2022-05-09T08:12:26Z
dc.date.available2022-05-09T08:12:26Z
dc.date.issued2018
dc.description.abstractBackground: Plasmodium falciparum malaria remains a major health challenge in Nigeria despite the global decline of its incidence and mortality rates. Although signifcant progress has been made in preventing the transmission of P. falciparum and controlling the spread of the infection, there is much to be done in the area of proper monitoring, surveillance of the parasite, investigating the population dynamics and drug resistance profling of the parasite as these are important to its eventual eradication. Polymorphic loci of msp1, msp2 and/or glurp genes or microsatellites have been traditionally used to characterize P. falciparum population structure in various parts of Nigeria. The lack of standardization in the interpretation of results, as well as the inability of these methods to distinguish closely related parasites, remains a limitation of these techniques. Conversely, the recently developed 24 single nucleotide polymorphism (SNP)-based molecular barcode assay has the possibility of diferentiating between closely related parasites and ofer additional information in determining the population diversity of P. falciparum within and between parasite populations. This study is therefore aimed at defning the population diversity of P. falciparum in and between two localities in Nigeria using the SNPs barcode technique. Methods: The 24-SNP high-resolution melt (HRM) barcode assay and msp2 genotyping was used to investigate both intra and inter population diversity of the parasite population in two urban cities of Nigeria. Results: Based on SNP barcode analysis, polygenomic malaria infections were observed in 17.9% and 13.5% of population from Enugu and Ibadan, respectively, while msp2 analyses showed 21% and 19.4% polygenomic infections in Enugu and Ibadan, respectively. Low levels of genetic diversity (π) of 0.328 and 0.318 were observed in Enugu and Ibadan parasite populations, respectively, while the FST value of 0.02 (p=0.055) was obtained when the genetic divergence of both populations was considered. Conclusions: The 24-SNP barcode assay was efective in analysing P. falciparum population diversity. This study also showed that P. falciparum populations in Enugu and Ibadan had a degree of intra-population diversity, but very low divergence between the population. A low degree of polygenomic infections were also observed in the two parasite populations unlike previous years. This maybe as a result of the efect of artemisinin-based combination therapy (ACT), long-lasting insecticide-treated nets (LLITNs) and intermittent preventive treatments in the study populations.en_US
dc.identifier.urihttp://dspace.run.edu.ng:8080/jspui/handle/123456789/2818
dc.language.isoenen_US
dc.publisherMalaria Journalen_US
dc.titleCharacterization of Plasmodium falciparum Structure in Nigeria with Malaria SNPS Barcode.en_US
dc.typeArticleen_US
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