Bioactivity assessment of exopolysaccharides produced by Pleurotus pulmonarius in submerged culture with different agro-waste residues
| dc.contributor.author | Oluwatoyin Modupe Aladejana | |
| dc.date.accessioned | 2025-05-23T18:19:15Z | |
| dc.date.available | 2025-05-23T18:19:15Z | |
| dc.date.issued | 2020-12-04 | |
| dc.description.abstract | Pleurotus spp. are white-rot fungi that utilize different agro-wastes to produce useful biologically active com pounds. In this study, exopolysaccharides (EPS) were produced by Pleurotus pulmonarius in submerged culture supplemented with different agro-wastes. Functional groups in EPS were revealed using Fourier Transform Infrared (FT-IR) spectroscopy. Antimicrobial activity of EPS was tested against microorganisms using agar well diffusion. Scavenging potentials of EPS was tested against 1, 1- diphenyl-2-picryhydrazyl (DPPH), hydroxyl (OH), iron (Fe2þ) and nitric oxide (NO) radicals. In vitro prebiotic activity of EPS was carried out. The highest yield (5.60 g/L) of EPS was produced by P. pulmonarius in submerged culture supplemented with groundnut shell (20.0 g/L). The functional groups in EPS were hydroxyl (-OH), methyl (-CH3), ketone (-RCOH) and carbonyl group (-C¼O). EPS displayed zones of inhibition (5.00–14.00 mm) against tested microorganisms. Scavenging activity of EPS ranged from 65.70-81.80% against DPPH. EPS supported the growth of Lactobacillus delbrueckii and Streptococcus thermophiles with values ranged from 3.04 104–3.40 104 cfu/ml and 2.50 104–2.81 104 cfu/ml, respectively. Submerged culture of P. pulmonarius with addition of agro-wastes enhanced yield of EPS. The EPS exhibited bio-functional properties like antimicrobial, antioxidant and prebiotic activities. Hence, agrowastes can be recycled in submerged fermentation with fungi to produce promising biomaterials for biopharmaceutical applications. | |
| dc.description.sponsorship | SELF | |
| dc.identifier.citation | Clement Olusola Ogidia,*, Adaeze Mascot Ubarub, Temilayo Ladi-Lawala, Oluwakemi Abike Thondab, Oluwatoyin Modupe Aladejanab, Olu Malomo (2020) | |
| dc.identifier.uri | https://doi.org/10.1016/j.heliyon.2020.e05685 | |
| dc.identifier.uri | https://repository.run.edu.ng/handle/123456789/4711 | |
| dc.language.iso | en | |
| dc.publisher | Heliyon | |
| dc.title | Bioactivity assessment of exopolysaccharides produced by Pleurotus pulmonarius in submerged culture with different agro-waste residues | |
| dc.type | Article |