Bioactivity assessment of exopolysaccharides produced by Pleurotus pulmonarius in submerged culture with different agro-waste residues
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Date
2020-12-04
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Heliyon
Abstract
Pleurotus spp. are white-rot fungi that utilize different agro-wastes to produce useful biologically active com
pounds. In this study, exopolysaccharides (EPS) were produced by Pleurotus pulmonarius in submerged culture
supplemented with different agro-wastes. Functional groups in EPS were revealed using Fourier Transform
Infrared (FT-IR) spectroscopy. Antimicrobial activity of EPS was tested against microorganisms using agar well
diffusion. Scavenging potentials of EPS was tested against 1, 1- diphenyl-2-picryhydrazyl (DPPH), hydroxyl (OH),
iron (Fe2þ) and nitric oxide (NO) radicals. In vitro prebiotic activity of EPS was carried out. The highest yield (5.60
g/L) of EPS was produced by P. pulmonarius in submerged culture supplemented with groundnut shell (20.0 g/L).
The functional groups in EPS were hydroxyl (-OH), methyl (-CH3), ketone (-RCOH) and carbonyl group (-C¼O).
EPS displayed zones of inhibition (5.00–14.00 mm) against tested microorganisms. Scavenging activity of EPS
ranged from 65.70-81.80% against DPPH. EPS supported the growth of Lactobacillus delbrueckii and Streptococcus
thermophiles with values ranged from 3.04 104–3.40 104 cfu/ml and 2.50 104–2.81 104 cfu/ml, respectively. Submerged culture of P. pulmonarius with addition of agro-wastes enhanced yield of EPS. The EPS exhibited bio-functional properties like antimicrobial, antioxidant and prebiotic activities. Hence, agrowastes can be recycled in submerged fermentation with fungi to produce promising biomaterials for biopharmaceutical
applications.
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Clement Olusola Ogidia,*, Adaeze Mascot Ubarub, Temilayo Ladi-Lawala, Oluwakemi Abike Thondab, Oluwatoyin Modupe Aladejanab, Olu Malomo (2020)