Cerebellar Cortex and the Behaviour of Mice (Mus Musculus) Following the Administration of Palm Wine
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Date
2020-08-07
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Abstract
Abstract: Aim:-To investigates the histomorphological studies of the
cerebellar cortex and the behaviour of mice after palm wine
administration. Material and Method:- 40 adult mice (25-30g) were
grouped into (A, B, C and D) comprised of 10 mice each. They were kept
in plastic cages and fed with feed and water ad libitum. Group A
(control group) received distilled water, group B ,C and D received
14.3ml/kg daily palm wine,14.3ml/kg palm wine every other day and
14.3ml/kg 50% diluted palm wine every other day respectively, orally for
four weeks. They were weighted using weighing balance, the
neurobehavioral evaluation was performed using the open field maze and
the health status was appraised by the rectal temperature. After the last
dose of administration, the animals were anaesthetized; the cerebellum
was excised, weighed and fixed in 10% formal saline for H&E, Cresyl
fast violet and bieschowsky silver stain techniques. Data were analyzed
using one way ANOVA at p<0.05 followed by a posthoc test, results
were expressed as mean S.E.M. Results;- decrease in the rectal
temperature of the entire treated group when compared to the control
group, relative weight of the cerebellum was higher in the treated group
compared to control, neurobehavioral observations (motor parameters)
showed difference between treated mice compared to control.
Histological observations showed vacuolation and slight degeneration of
Purkinje neuron cell bodies except in group D animals. Conclusion:- The
study showed that palm wine intake on regular basis may affect the
histoarchitecture of the cerebellum, neurobehavioural activities such as
locomotion and balancing