Decolorization of Dye House Effluent and Biodegradation of Congo red by Bacillus thurengesis RUN1
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Date
2013
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Publisher
Journal of Microbiology and Biotechnology,
Abstract
A dye-decolorizing bacterium was isolated from a soil
sample and identified as Bacillus thuringiensis using 16S
rRNA sequencing. The bacterium was able to decolorize
three different textile dyes, namely, Reactive Blue 13,
Reactive red 58, Reactive yellow 42, and a real dyehouse
effluent up to 80-95% within 6 h. Some non-textile
industrially important dyes were also decolorized to
different extents. Fourier transform infrared spectroscopy
and gas chromatography-mass spectrometer analysis of the
ethyl acetate extract of Congo red dye and its metabolites
showed that the bacterium could degrade it by the
asymmetric cleavage of the azo bonds to yield sodium (4-
amino-3-diazenylnaphthalene-1-sulfonate) and phenylbenzene.
Sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) was
further oxidized by the ortho-cleavage pathway to yield 2-
(1-amino-2-diazenyl-2-formylvinyl) benzoic acid. There
was the induction of the activities of laccase and azoreductase
during the decolorization of Congo red, which suggests
their probable role in biodegradation. B. thuringiensis
was found to be versatile and could be used for industrial
effluent biodegradation.
Description
Keywords
Decolorization, dyehouse effluent, Congo red, UV-visible analysis, GC-MS analysis