Polyphenol-rich extract of Vernonia amygdalina (Del.) leaves ameliorated cadmium-induced alterations in feeding pattern and urine volume of male Wistar rats
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Date
2015-11-13
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ScopeMed
Abstract
Aim: To determine the effects of polyphenol-rich extract of the leaves of Vernonia amygdalina (PEVA) on the
feeding pattern of rats that were exposed to cadmium (Cd) toxicity. Materials and Methods: Thirty male
Wistar rats, weighing 160-180 g, were divided into 6 groups of 5 rats each as follows; Group 1 received distilled
water orally (0.2 ml/100 g), daily, throughout the period of study. Group 2 received Cd alone (in the form of
CdSO4) at 5 mg/kg/day via intraperitoneal route for 5 consecutive days. Group 3 were pre-treated with Cd as
Group 2 and thereafter left untreated for a period of 4-week. After the oral lethal dose of PEVA was determined,
Groups 4, 5, and 6 were pre-treated with Cd as Group 2 after which they received graded doses of PEVA at
100, 200 and 400 mg/kg/day (0.2 ml/100 g), respectively via oral route for 4 weeks. Blood samples were
collected for some plasma biochemical assays while urine samples were collected using metabolic cages.
Results: PEVA administration significantly increased (P < 0.05) the body weight and feeding patterns that
were significantly reduced (P < 0.05) by Cd toxicity. PEVA also significantly reinstated the plasma antioxidant
status, as well as glucose and urine volume of the rats toward control values (P < 0.05). Conclusion: PEVA
can be an herbal alternative in the treatment or management of subjects manifesting alterations in feeding
pattern and urine volume that is Cd-induced