Clerodendrum volubile Ethanol Leaf Extract: A Potential Antidote to Doxorubicin-Induced Cardiotoxicity in Rats
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Date
2020-07-04
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Journal of Toxicology
Abstract
Doxorubicin is widely applied in hematological and solid tumor treatment but limited by its off-target cardiotoxicity. us,
cardioprotective potential and mechanism(s) of CVE in DOX-induced cardiotoxicity were investigated using cardiac and ox
idative stress markers and histopathological endpoints. 50–400mg/kg/day CVE in 5% DMSO in distilled water were investigated
in Wistar rats intraperitoneally injected with 2.5mg/kg DOX on alternate days for 14days, using serum troponin I and LDH,
complete lipid profile, cardiac tissue oxidative stress marker assays, and histopathological examination of DOX-treated cardiac
tissue. Preliminary qualitative and quantitative assays of CVE’s secondary metabolites were also conducted. Phytochemical
analyses revealed the presence of flavonoids (34.79±0.37mg/100mg dry extract), alkaloids (36.73±0.27mg/100mg dry extract),
reducing sugars (07.78±0.09mg/100mg dryextract), and cardiac glycosides (24.55±0.12mg/100mg dry extract). 50–400mg/kg/
day CVEsignificantly attenuated increases in the serum LDH and troponin I levels. Similarly, the CVE dose unrelatedly decreased
serum TG and VLDL-c levels without significant alterations in the serum TC, HDL-c, and LDL-c levels. Also, CVE profoundly
attenuated alterations in the cardiac tissue oxidative stress markers’ activities while improving DOX-associated cardiac histo
logical lesions that were possibly mediated via free radical scavenging and/or antioxidant mechanisms. Overall, CVE may play a
significant therapeutic role in the management of DOX-induced cardiotoxicity in humans.