Pterocarpus mildbraedii leaf extract ebbs Propanil-induced Oxidative and Apoptotic Damage in the Liver of Rats
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Date
2020-11-04
Authors
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Taylor and Franscis
Abstract
Phytochemicals derived from plant sources are well recognized as sources of pharmacologically potent
drugs in the treatment of several oxidative stress-related ailments. Dichloromethane/methanol (1:1) leaf
extract of Pterocarpus mildbraedii was evaluated for its possible protection against oxidative stress and
apoptosis in the liver of male Wistar rats exposed to propanil (PRP). In the experimental design, olive
oil served as the vehicle, and rats were grouped into control (2 mL/kg olive oil), PRP (200mg/kg/day),
Pterocarpus mildbraedii extract (200 mg/kg/day), and Pterocarpus mildbraedii extract (200mg/kg/
day)þPRP (200 mg/kg/day), and treated daily, p.o., for seven days. Oxidative stress parameters, B-cell
lymphoma 2 (Bcl-2), Bcl 2-associated X protein (Bax), p53, caspases (9/3), and terminal transferase dUTP
nick end labeling (TUNEL) assays were observed in all groups. Propanil significantly elevated superoxide
dismutase and lipid peroxidation levels, while concomitantly depleting GSH and p53 levels. Further,
PRP enhanced the expressions of caspase-9, caspase-3, Bax, and TUNEL-positive cells in the liver of rats.
However, these observed alterations were reversed following treatment with Pterocarpus mildbraedii
extract. Our studies suggest that Pterocarpus mildbraedii extract protected against PRP toxicity by
reducing oxidative stress and attenuating critical endpoints in the intrinsic apoptotic pathway.
Description
Keywords
Apoptosis, TUNEL Assay
Citation
Otuechere, C.A. and Farombi, E.O. (2020). Pterocarpus mildbraedii leaf extract ebbs propanil-induced oxidative and apoptotic damage in the liver of rats. Drug and Chemical Toxicology. https://doi.org/10.1080/01480545.2020.1842884.