Growth of Raphia farinifera and Elaeis guineensis Wine Yeast on Cassava (Manihot esculenta) and Poultry Manure Media
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Date
2014
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Publisher
Nigerian Society for Experimental Biology
Abstract
The aim of this research was to determine the suitability of cassava (Manihot esculenta) and poultry manure
(locally available resources) as substrates for the production of raffia and palm wine yeasts. Yeast growth was
estimated as a measure of their medium’s optical density at 600 nm (OD600 nm) after 36 h of growth. The various media used were formed from different combinations of cassava flour and poultry manure. The results obtained showed that the growth of the palm wine (Elaeis guineensis Jacq.) yeast in the locally formulated media 3:2, 4:1, 5:0 [poultry manure (PM)(g)/ cassava flour (CS)(g)], and yeast extract-peptone dextrose medium (YPD) was 2.101, 2.830, 3.131 and 2.057 respectively. The OD600 nm measurement of the growth of the raffia wine (Raphia farinifera) yeast was 2.912, 2.859, 4.000, and 2.110 respectively in the same media above. This shows that formulations 3:2, 4:1, and 5:0 [PM (g)/CS (g)] are highly suitable for the growth of palm wine and raffia wine yeasts when compared with their growth in the standard medium (YPD). It was also an indication that they contain adequate amounts of reducing sugar and protein which served as sources of carbon and nitrogen respectively, for the growth of the two yeasts (palm wine and raffia wine yeasts). The results of this study indicate that cassava and poultry manure are suitable substrates for yeast production.
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Keywords
African oil palm (Elaeis guineensis Jacq.), Raffia palm (Raphia farinifera), Yeast (Saccharomyces cerevisiae), Growth media, Poultry manure, Cassava (Manihot esculenta) flour