Antimicrobial Activities of Extracts of Macrosphyra longistyla against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps
| dc.contributor.author | Folarin, Onikepe | |
| dc.date.accessioned | 2025-05-28T10:46:33Z | |
| dc.date.available | 2025-05-28T10:46:33Z | |
| dc.date.issued | 2024-03-13 | |
| dc.description.abstract | The world will benefit from more effective antimicrobial agents against oral conditions arising from the actions of biofilm forming bacteria. Also, infor mation is lacking on the oral biofilm-forming bacterial diversity in South western Nigeria. In this study, we isolate and characterize oral biofilm pro ducing bacteria in the oral cavities of schoolchildren in Southwestern Nigeria. We also investigate the antimicrobial properties of Macrosphyra longistyla extracts against the biofilm-formers and the toxicity of potent extracts. Sam ples were obtained from 109 schoolchildren aged 4 - 14 years from Lagos, Oyo and Osun States. Agar well diffusion technique was used in the antimi crobial susceptibility testing. Toxicity testing was done using brine shrimps (Artemia salina). Biofilm-formers in this study are Klebsiella sp., Streptococ cus sp., Staphylococcus sp., and Micrococcus sp. Ethanol leaf extracts had the highest activity against all biofilm-producing bacteria. Ethanol stem bark ex tract, which elicited activity against Klebsiella only, was found to be less toxic than the ethanol leaf extract. Staphylococcus showed >10 mm susceptibility to the ethanol and aqueous extracts of Macrosphyra longistyla. Streptococcus and Micrococcus were susceptible to the antimicrobial actions of the ethanol ic leaf extracts. Although the ethanol extracts of the leaves had lower mini mum inhibitory concentrations than the ethanol extracts of the stem bark, toxicity studies showed ethanol extracts of the stem-bark to be more toxic than the ethanol extracts of the leaves. In conclusion, ethanolic extracts of Macrosphyra longistyla show potential as sources of antimicrobials against gram-positive, oral biofilm-forming bacteria. Keywords Biofilms, Plant Extracts, Toxicity, Bacteria, Susceptibility, Antimicrobial | |
| dc.identifier.uri | 10.4236/aim.2024.143012 | |
| dc.identifier.uri | https://repository.run.edu.ng/handle/123456789/4886 | |
| dc.language.iso | en | |
| dc.relation.ispartofseries | 13 | |
| dc.title | Antimicrobial Activities of Extracts of Macrosphyra longistyla against Gram-Positive Oral Biofilm-Formers from School Children in Southwestern Nigeria and Toxicity Studies Using Brine Shrimps | |
| dc.type | Article |