Basic Principles of Fluorescence Microscopy
| dc.contributor.author | Omotoso, Dayo | |
| dc.date.accessioned | 2022-05-25T09:06:46Z | |
| dc.date.available | 2022-05-25T09:06:46Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Fluorescence microscopy is a basic requirement in cell biology , molecular biology and biotechnology . Advancements over the years has helped scientist to trace molecules in live cells and understand the basis of cell metabolism, exchange, mutation and toxicity. In this short communication we seek to explain in simple terms the basic principles of how a fluorescence microscope works. The principles of excitation and emission focuses on the ability of fluorophores to absorb energy from photons and to emit such absorbed energy. The difference between the chemical structures of these fluorephores determines how much energy that is required to excite them and how long a fluorescence signal from a fluorophore will last. The principles of epi-illumination on the other hand describe the arrangement and function of the various components of a fluorescence microscope. | en_US |
| dc.identifier.issn | 2249-0566 | |
| dc.identifier.uri | http://dspace.run.edu.ng:8080/jspui/handle/123456789/3237 | |
| dc.language.iso | en | en_US |
| dc.publisher | World J Young Researchers | en_US |
| dc.subject | Microscopy | en_US |
| dc.subject | Fluorescence | en_US |
| dc.subject | Light | en_US |
| dc.title | Basic Principles of Fluorescence Microscopy | en_US |
| dc.type | Article | en_US |