Assessment of Gastroprotective Activity of Aqueous Leaf Extract of Ageratum conyzoides L.: Role of Mucous Cells, Anti-apoptotic (Bcl-2) and Tumor Suppressor (p53) Proteins
| dc.contributor.author | Omotoso, Dayo | |
| dc.date.accessioned | 2022-04-30T13:36:00Z | |
| dc.date.available | 2022-04-30T13:36:00Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Introduction: Generally, factors that up-regulate gastric mucosal protective factors or downregulate aggressive factors contribute to the maintenance of mucosal integrity. This study was done to assess the role of mucous cells, Bcl-2 and p53 proteins during the gastroprotective activity of aqueous extract of Ageratum conyzoides. Methods: The phytochemistry of A. conyzoides extract was analyzed using a gas chromatographymass spectrometer. Animals were subdivided into five groups, including non-treated normal control group A, non-treated test control group B, and treated groups C-E (Pre-treated with 100, 300, and 500 mg/kg A. conyzoides, respectively for 28 days). After the treatment period, pyloric-ligation was used to induce mucosal injury. Gastric tissues were harvested, grossly examined, and processed for histological, histochemical, and immunohistochemical studies. Stained sections were examined and quantified using image-J software. The data were analyzed using IBM-SPSS (version 23), and comparisons were checked via t test and analysis of variance. Results: Mild mucosal erosion was observed in the treated groups, but intense erosion was prominent in the test control animals. There was an insignificant increase in mucous cells, a significant (P < 0.05) increase in Bcl-2 expression without a significant increase in p53 expression in gastric mucosa of pre-treated animals compared to normal control. Gastric mucosa of test control showed a significant (P < 0.05) decrease in mucous cell count and Bcl-2 expression with a significant concomitant increase in p53 expression. Conclusion: Increased mucous cell population and reciprocal expressions of Bcl-2 and p53 proteins in the gastric mucosa of animals highlighted the sub-cellular mechanisms of gastroprotective activity of A. conyzoides. | en_US |
| dc.identifier.uri | http://dspace.run.edu.ng:8080/jspui/handle/123456789/2576 | |
| dc.language.iso | en | en_US |
| dc.subject | Gastroprotection | en_US |
| dc.subject | Ageratum conyzoides | en_US |
| dc.subject | Mucous cells | en_US |
| dc.subject | Apoptotic markers | en_US |
| dc.title | Assessment of Gastroprotective Activity of Aqueous Leaf Extract of Ageratum conyzoides L.: Role of Mucous Cells, Anti-apoptotic (Bcl-2) and Tumor Suppressor (p53) Proteins | en_US |
| dc.type | Article | en_US |