Nuerobehavioural Investigation of The Role/S of Moringa Oleifera On 3-Nitropropionic Acid Model of Huntington’s Disease
Loading...
Date
2019-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Background: Huntington’s disease (HD) is a neurodegenerative
disorder characterized by motor impairment that contribute to
the death of striatial neurons and psychiatric symptoms.
Systemic administration of 3-Nitropropionic Acid (3-NP) cause
selective striatial degeneration similar to that seen in HD. Recent
studies clearly demonstrate that increase oxidative stress is one
of the major deleterious even in the 3-NP-induced
neurodegenerative process. Moringa oleifera (MO) has been
studied for the anti-oxidant, anti-inflammatory properties. This
study evaluated the Neurobehavioral role of MO following 3-NP
model of Huntington's disease.
Methodology: Thirty-two adult mice weighting between 25-32g
were used and divided into four groups (A, B, C and D), Group
A serves as the control (Received food and ad libitum), B
(Received MO+3-NP), C (Received only 3-NP) and D (Received
3-NP + MO) 3-Nitropropionic Acid (20mg/kg for 7 days) were
induced intraperitoneal to the animals. MO were administer
orally (250mg/kg) for a period of 7 days. Weights of the animals
were checked periodically using the Acucuscope weighing
Balance. Neurobehavioral assessment was carried out after
administration. Animals were sacrificed at the end of the
experiment Neurotransmitters- Dopamine and Glutamate were
assayed in tissue homogenates and the levels were quantified.
Results showed increased tissue levels of glutamate and
dopamine and impaired neurobehavioural parameters in the
Huntington's group, while the preventive group showed some
degree of preservation of the normal parameters.
Conclusion: 3-Np induced huntington's disease model in mice as
expected with the characteristics of increased tissue levels of
glutamate and dopamine and impaired behavioural and
locomotive indices.
Description
Keywords
indices. Key words: huntington's disease, 3-Nitropropionic acid, Moringa, Neurobehavioral & Neurotransmitter