Identification of bacterial pathogens isolated from smoked blue whiting fish (Micromesistius poutasou) from Odeomu market in Osun state Nigeria
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Date
2023-08-12
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German Journal of Veterinary Research
Abstract
The presence of microorganisms is one of the major factors affecting the quality of
smoked fish sold in the open markets. Smoked blue whiting fish (Micromesistius pouta
sou), commonly called (Panla) sold in the Odeomu market in Osun State, were ana
lyzed for microbial contaminants. Isolates were identified using conventional biochemical
methods, and antibiotics susceptibility testing was carried out using the disc diffusion
method. The total bacterial counts (TBC) results showed that the fish samples had high
bacterial counts, ranging from 2.1×103 to 9.2×103 colony-forming units (CFU)/g. Bac
teria isolated from the fish samples were: E. coli (45.46%), Enterobacter spp. (1.01%),
Klebsiella spp. (6.06%), Proteus spp. (9.09%), Salmonella spp. (7.07%), Shigella spp.
(19.19%), Bacillus spp. (4.04%) and Staphylococcus spp. (8.08%). The antibiotic sen
sitivity pattern of Gram-Negative bacteria indicated that all the isolates were resis
tant to more than three antibiotics. All E. coli isolates were resistant to augmentin
and ceftazidime, 82.2% were resistant to cefuroxime, 17.7% to gentamicin, and 6.7% to
ofloxacin. Screening of resistance genes showed that all six selected multiple antibiotic
resistant E. coli isolates tested harbored TEM gene, and two isolates (33.33%) harbored
the aac (3)-II gene. None of the isolates harbored SHV, CTX-M, and qnrB genes. Our
results showed that smoked blue whiting fish may pose a significant risk of spreading
antibiotic-resistant bacteria that contain multiple antibiotic-resistance genes, highlight
ing a serious public health concern.
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Oluwatoyin M. Aladejana, Mary Zakka, Ayodele O. Ogunlade and Oluwakemi Abike Thonda (2023)