Microbiological assessment and Multiple Antimicrobial Resistance Profile of Enterobacteriaceae recovered from kulikuli sold at selected markets in Ife-North Local Government Area
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Date
2023-08-09
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Nigerian Journal of Pure & Applied Science
Abstract
The native West African population eats a groundnut-based snack called kulikuli (groundnut cake). The study was
carried out in order to evaluate, characterize, and determine multiple antibiotic resistance index (MAR-I) of isolated
pathogen isolated in ready-to-eat kulikuli snacks. 52 samples of kulikuli in total were collected for this investigation
from Odeomu market and its surroundings in Osun State, Nigeria. Using the pour plate technique, bacteriological
analysis of the samples was performed. Total coliform counts were counted on MacConkey agar, total bacterial
counts on Nutrient agar, and total fungal counts were counted on potato dextrose agar. Phenotypic and biochemical
assays were used in identifying the bacterial isolates. Disc diffusion techniques were used to test the isolates'
antibiotic susceptibility, and the MAR index of the isolates was determined. The total bacterial count, total coliform
count and the total fungal count of the samples varied from 1.5 to 8.8×103cfu/g, 0.3 to 6.6×103cfu/g and 1 to
7.3×104cfu/g respectively. The organisms recovered were enteric, Gram-negative members of the Enterobacteriaceae
family. Multiple antibiotic resistance (MAR) to four to seven different antibiotics was developed by the isolates. The
research area has a high overall rate of multiple drug resistance pathogens isolated from the kulikuli samples. The
MAR Index was higher than 0.2, however a MAR index that is greater than 0.2 suggested high-risk sources, like
places where bacteria have been repeatedly exposed to antibiotics or where the use of antibiotic is common. In
conclusion, in order to avoid consuming these pathogens and harmful toxins together with the products, kulikuli
should be sold in hygienic conditions and environments, be sealed, and not be exposed to air. This is because the
organisms identified in this study indicated health risks that would be harmful to consumers.
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Thonda O.A., Wilkie, E.D., Ogidi, C.O., Aladejana, O.M. and Degan, B. (2023)