Curcuma longa Extracts Suppress Pathophysiology of Experimental Hepatic Parenchymal Cell Necrosis
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Date
2019-04-14
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Publisher
Elsevier
Abstract
tThe study sought to investigate the protective potentials of Curcuma longa rhizome following potassiumbromate-induced liver injury in Wistar rats. Thirty-five male Wistar rats were divided into 7 groups of 5rats each (n = 5). Control group received normal saline while the other groups received oral administra-tion of 100 mg/kg potassium bromate daily for two weeks to induce hepatic injury. Negative control I ratswere sacrificed immediately after induction of hepatic injury, while the test groups were given oral doseof ethanol extract of Curcuma longa rhizome (EECLOR) at 100, 200 and 400 mg/kg for two weeks. Positivecontrol group was treated with Silymarin for two weeks, while negative control II group was observed forthe two-week period. At the end of the study, serum biochemical parameters of liver function enzymes,malondialdehyde and histopathological changes were investigated. Necrotic hepatocytes were quantifiedin H&E-stained liver sections using the morphologic criteria of typical necrotic tissue. Hepatocytes thatremained intact were identified as those with round euchromatic nuclei with prominent nucleoli. Histo-logical examination and morphological grading of the stained sections showed massive necrosis acrossthe zones. EECLOR improved liver functions evidenced by reduced activity of serum amino transferases. Italso reduced lipid peroxidation. In addition, there was significant reduction of hepatocytes showing mor-phological criteria of necrosis in EECLOR-treated rats across the zones, with appreciable radial sinusoidalarrangement. In conclusion, the protective actions of EECLOR against potassium bromate liver toxicity inrats, appears to be due to its ability to reduce lipid peroxidation.
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Keywords
Potassium bromate, Curcuma longa, Liver injury, Massive necrosis, Karyorrhexis, Lipid peroxidation
Citation
Adeyemi DO, Awoniran PO. Curcuma longa extracts suppress pathophysiology of experimental hepatic parenchymal cell necrosis. Pathophysiology. 2019 Jun;26(2):153-162. doi: 10.1016/j.pathophys.2019.04.002. Epub 2019 Apr 16. PMID: 31023565.