Degradation of Indigo Dye by a Newly Isolated Yeast, Diutina Rugosa from Dye Wastewater Polluted Soil
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Date
2017-09
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Elsevier
Abstract
Isolation, identification, and characterization of newly isolated yeast, Diutina rugosa capable of decolorizing
indigo dye were investigated in this study. Molecular and phylogenetic analyses of 23S rRNA sequence data
indicated that the yeast belonged to the new genus, Diutina. The optimization of physicochemical parameters
such as pH of the solution (2–8), initial dye concentration (10–60 mg L−1), adsorbent mass (0.1–2 g), and
temperature (10–50 °C) was studied to scale- up the conditions of dye removal. Furthermore, complete decolorization (99.97%) of indigo dye (10 mg L−1) was achieved at pH 2, temperature 30 °C and 2.0 g cell biomass within 5 days. Degradation was monitored through UV–vis spectrophotometric, FTIR, and GCMS analyses. The results of FTIR analysis obtained revealed the loss and shifts in spectra peaks of the experimental in comparison with the biological control. Possible degradation pathway was proposed using the intermediate metabolites; 1, 2-dihydro-3H-indol-3-one and cyclopentanone obtained through GCMS analysis. The enzyme analyses revealed significant inductions and major roles played by NADH-DCIP reductase and lignin peroxidase in the asymmetric cleavage, initial reduction and deamination of indigo dye. The equilibrium experimental data were fitted to Langmuir, Freundlich and Temkin adsorption Isotherms with high adjusted coefficient of determination values; adjR2 =0.907, adjR2 =0.867, and adjR2 =0.965 respectively. However, the Langmuir and Temkin adsorption isotherms affirmed the monolayer and heterogeneous biosorption characteristics of Diutina rugosa. Temkin adsorption isotherm model (R2 =0.971) represented the best fit of experimental data than other isotherm models.
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Keywords
Biodegradation, Indigo dye, Yeast, Diutina rugosa, Adsorption isotherms
Citation
Journal of Environmental Chemical Engineering 5 (2017) 4639–4648