Isolation, Identification and in silico Analysis of Alpha-amylase Gene of Aspergillus niger strain CSA35 Obtained from cassava Undergoing Spoilage

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dc.contributor.authorAvwioroko, Oghenetega
dc.date.accessioned2022-09-15T12:15:07Z
dc.date.available2022-09-15T12:15:07Z
dc.date.issued2018
dc.description.abstractIn this investigation, a gene (CDF_Amyl) encoding extracellular α-amylase in Aspergillus niger strain CSA35 associated with cassava spoilage was amplified using specific primers and characterized in silico. The gene had a partial nucleotide sequence of 968 bp and encoded a protein of 222 aa residues with a molecular weight and isoelectric point of 25.13 kDa and 4.17, respectively. Its catalytic site was located in the active site domain. BLASTp analysis showed that the protein primary sequence of the α amylase gene had 98% and 99% homologies with the α-amylase of A. niger and A. oryzae RIB40, respectively. The gene is more closely related to α-amylase genes from fungi than to bacterial, plant, or animal α-amylase genes. Restriction mapping of the gene showed it can be digested with restriction enzymes like NcoI, PstI, SmaI, and BcLI among others but not with EcoRI and EcoRV. Its protein product had a hydrophobicity score of − 0.43 but no transmembrane helix. The CDF_Amyl protein was subcellularly localized in the secretory pathway, an indication of its release into extracellular space after secretion. Also, the 3D structure of the CDF-Amyl protein was barrel-shaped with domains characteristic of α-amylases. The encoded α-amylase Vmax is 6.90 U/mg protein and Km is 6.70 mg/ml. It was concluded that the unique characteristics of the CDF_Amyl gene and its deduced protein could find applications in biotechnological, food and pharmaceutical industries where cloning and further modification of this gene would be required for product development and improvement.en_US
dc.identifier.issnhttps://doi.org/10.1016/j.bbrep.2018.03.006
dc.identifier.urihttp://dspace.run.edu.ng:8080/jspui/handle/123456789/3635
dc.language.isoenen_US
dc.publisherELSEVIER Biochemistry and Biophysics Reportsen_US
dc.subjectAspergillus nigeren_US
dc.subjectAlpha-amylase geneen_US
dc.subjectBioinformatics characterizationen_US
dc.subjectRestriction enzymes Phylogeneticsen_US
dc.subjectEvolutionen_US
dc.titleIsolation, Identification and in silico Analysis of Alpha-amylase Gene of Aspergillus niger strain CSA35 Obtained from cassava Undergoing Spoilageen_US
dc.typeArticleen_US
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