Biochemical profiling of albino wistar rats (Rattus norvegicus) fed imarsilTM - treated aflatoxin M1 (AFM1) contaminated milk

Abstract

The potential of imarsilTM to decontaminate aflatoxin M1 (AFM1) from cow’s milk has been documented in previous study. The present study focused on biochemical profiling of albino wistar rats (Rattus norvegicus) fed ImarsilTM - treated AFM1 contaminated milk. Seventy-two male Wister albino rats were randomly allocated to four treatment groups (A – D) in a completely randomized design with three replicates of six rats each in a six weeks study. The treatments given to the four groups were as follows: Group A-Rats fed with standard ration and 2 ml of clean distilled water; Group B- Rats fed with standard ration and 2 ml of milk; Group C-Rats fed with standard ration and 2 ml of AFM1 contaminated milk (456 ng/L); Group D- Rats fed with standard ration and 2 ml of AFM1 contaminated milk (456 ng/L) treated with imarsilTM at 2% dosage rate. Feeding AFM1 contaminated milk (456 ng/L) to male albino rats elicited abnormal levels of alanine aminotransferase, serum albumin, creatinine, and blood calcium. However, addition of imarsil at 2% dosage rate to the contaminated milk provoked protective efficacy against the hepatotoxicity of AFM1 and normalization of blood calcium level. Therefore, imarsil represents an effective and safe adsorbent for the remediation of AFM1 contaminated milk in the tropical developing world.