Phytochemical Screening and Antibacterial Activity of Two Plant Extracts against Multidrug-Resistant Stenotrophomonas maltophilia of Clinical Origin.
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Date
2023
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Journal Title
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Publisher
Nigerian Journal of Microbiology
Abstract
New antibacterial agents are pressingly in need to combat the outgrowing incidence of bacterial
resistance, which has been number one significant public health threat. The antibacterial activity and
phytochemical
components
of Cannabis
sativa and Garcinia
kola against Stenotrophomonas
maltophilia from diabetic patients with wound infection were investigated. Pure and type isolates of S.
maltophilia (ATCC 17666) were obtained from Ogun and Lagos State hospitals, while the plants used
were purchased from Ago-Iwoye market, Ogun State. Disk and agar diffusion methods were used to
evaluate the antibacterial profile, and the effect of the extracts on the studied isolates, respectively. The
isolates of S. maltophilia were resistant to all the antibiotics tested. The effect of the two extracts on the
two S. maltophilia varied based on the plant solvent, concentration, and organisms. Ethanol extracts of G.
kola and C. sativa on pure/type S. maltophilia had the highest diameter zones of inhibition of 24 mm/27
mm and 19 mm/22 mm at 150 mg/ml, respectively. The type isolate had the highest diameter zones of
inhibition in all concentrations of the solvent extracts except for the aqueous. Minimum inhibitory and
bactericidal concentration for both plants were 75 mg/ml (type isolate), 150 mg/ml (pure isolate), and 75
mg/ml for C. Sativa alone. Some phytochemicals such as alkaloids, flavonoids, tannins, saponins were
observed in the plant extracts.In conclusion, the tested plants possess antibacterial activity, hence, could be
used as substitutes in the treatment and management of S. maltophilia pathogens in diabetics with wound
infection, and also, in the formulation of pharmaceuticals.