First report of Wolbachia from field populations of Culex mosquitoes in south-western Nigeria

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dc.contributor.authorFemi Ayoade
dc.date.accessioned2025-06-02T14:17:20Z
dc.date.available2025-06-02T14:17:20Z
dc.date.issued2019-10-16
dc.description.abstractRecent reports on finding Wolbachia-strain infections in field mosquito species in some West African countries and the potential for developing these as disease vector biocontrol tools have prompted a search for Wolbachia in mosquitoes within the study area. Using a completely randomised design, mosquito traps were set at different locations in a rural and an urbanised community. One hundred and eighty (180) mosquitoes were trapped and pooled on the basis of genus, sex and site of collection, because there have been no earlier reports of Wolbachia isolated from Nigeria. Twenty pools, made up of not more than ten mosquitoes per pool, were homogenised and analysed for Wolbachia-specific DNA. Mosquitoes were trapped within Ede (urbanised community) and Akoda (rural community). Genomic DNA was extracted from trapped mosquito samples and used as a template in a PCR reaction. The Wolbachia sp. specific 16S rRNA gene was amplified, sequence analysis of PCR products was performed and a chromatogram of the sequence was subjected to Basic Local Alignment Search Tool analysis to identify the Wolbachia sp. This sequence was subsequently submitted to GenBank with accession number MK127541. The first evidence of the presence of the endosymbiont, Wolbachia in field-caught mosquitoes is hereby documented. The homology of this strain of Wolbachia bears similarities to those reported recently from other parts of West Africa and forms a single clade with a Wolbachia sp. from Mali, with a strong bootstrap support of 99%. This finding of a Wolbachia strain in mosquitoes at Ede could form the basis for more searches for diverse strains of Wolbachia in Nigeria.
dc.description.sponsorshipThis study was supported by grants from the National Institute of Allergy and Infectious Diseases, NIH-H3Africa (U01HG007480 and U54HG007480 to Dr Christian Happi and a grant from the World Bank (project ACE019 to Redeemer’s University - Dr Christian Happi).
dc.identifier.citationTosin Segun Ogunbiyi, Philomena Eromon, Paul Oluniyi, Femi Ayoade, Owoicho Oloche, Judith Uche Oguzie, Onikepe Folarin, Christian Happi & Isaac Komolafe (2019) First report of Wolbachia from field populations of Culex mosquitoes in south-western Nigeria, African Zoology, 54:3, 181-185, DOI: 10.1080/15627020.2019.1649092
dc.identifier.uri10.1080/15627020.2019.1649092
dc.identifier.urihttps://repository.run.edu.ng/handle/123456789/5144
dc.language.isoen
dc.relation.ispartofseries54; 3
dc.titleFirst report of Wolbachia from field populations of Culex mosquitoes in south-western Nigeria
dc.typeArticle
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