In Vivo Hepatotoxicity of Chemically Modified Nanocellulose in Rats
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Date
2020-02-13
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Publisher
Sage
Abstract
Chemical modification of cellulose is currently attracting attention as researchers attempt to take advantage of
the abundance of hydroxyl groups on its surface to introduce extra biological functionality. However, the
possible deleterious effect of exposure to functionalized nanocellulose (CSN) remains a concern. Therefore,
this study aims to explore the potential mechanisms of hepatotoxicity of CSN modified with oxalate ester
(NCD) in rats. A 7-day repeated oral toxicity study of NCD at the doses of 50 and 100 mg kg1 body weight
was conducted, and plasma and liver tissue samples were assayed using biochemical analysis, liver histopathology, and protein expression. NCD, at both doses, did not significantly (p > 0.05) alter the relative weight of
liver, alkaline phosphatase activity, and lipid peroxidation levels of the animals. However, NCD at the dose of
100 mg kg1 body weight significantly elevated aspartate aminotransferase, alanine aminotransferase, and
myeloperoxidase activities. NCD also enhanced the immunohistochemical expression of inducible nitric oxide
synthase and Bcl-2-associated X protein in the liver of rats. Histological observations revealed necrosis and
severe cellular infiltration at the high-dose treatment. Our study provides an experimental basis for the safe
application of NCDs.
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Keywords
Immunohistochemistry, Nanocellulose oxalate ester, Liver, Toxicity testing, Rats
Citation
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