Molecular characterization of extended spectrum β-lactamase producing- Enterobacteriaceae from bats (Eidolon helvum) faeces in Osun State, Nigeria
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Date
2021-04-21
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BIOLOGICA NYSSANA
Abstract
Enterobacteriaceae from faecal samples of Eidolon helvum were isolated and
identified biochemically then further confirmed using Analytical Profile Index
20E Kit. Antibiotic susceptibility testing and extended spectrum b-lactamase
(ESBL) production using Mast Disc were carried out. Detection of CTX-M
gene was done using polymerase chain reaction (PCR) employing appropriate
primers. A total of 337 bacterial isolates was recovered from the studied
locations consisting of forty-one species belonging to fifteen genera. Species
of Citrobacter, Enterobacter, Salmonella, Klebsiella spp, and Escherichia
coli were the most abundant and common to the three studied locations, with
Salmonella spp being the most predominant. A total of 35.9% of the selected
isolates was produced ELBS and 14.28% of the isolates harbours CTX-M
gene. The study concludes that E. helvum in the study areas are reservoirs of
Enterobacteriaceae and some harboured CTX-M gene which confirmed their
pathogenicity with public health consequences
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Oluwatoyin Modupe Aladejana and Anthonia Olufunke Oluduro