Resourcefulness of propylprodigiosin isolated from Brevundimonas olei strain RUN‑D1
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Date
2023-07-09
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AMB Express
Abstract
A novel red-pigmented bacterium was isolated from a water sample collected at Osun River, Ede. Morphological
and 16 S rRNA gene sequencing revealed that the bacterium is a strain of Brevundimonas olei, while its red pigment
was identified using UV-visible, FTIR and GCMS as a derivative of propylprodigiosin. The maximum absorbance
of 534 nm, the FTIR’s 1344 cm−
1 peak of prodigiosin’s methoxyl C-O interaction, and the molecular ions from GCMS
confirmed the pigment’s identity. The pigments production was temperature-sensitive (25 °C), lost at > 28 °C,
and in the presence of urea and humus. In addition, the pigment turned pink in the presence of hydrocarbons,
while its red colour was retained with KCN and Fe2SO4,
and enhanced by methylparaben. Furthermore, the pigment
is stable in high temperature, salt, and acidic conditions, but changed to yellow in alkaline solution. The pigment,
identified as propylprodigiosin (m/z 297), demonstrated broad-spectrum antibacterial activities against clinically
important strains of Staphylococcus aureus (ATCC25923), Pseudomonas aeruginosa (ATCC9077), Bacillus cereus
(ATCC10876), Salmonella typhi (ATCC13311), and Escherichia coli (DSM10974). The ethanol extract has the highest
zones of inhibition of 29 ± 3.0, 26 ± 1.2, 22 ± 3.0, 22 ± 1.5, and 20 ± 2.0 mm, respectively. Furthermore, the acetone pigments
interacted with cellulose and glucose such that increasing glucose concentrations showed linearity at 425 nm.
Finally, the fastness of the pigments to fabrics was excellent, with percentage fadedness of 0 and − 43% light
and washing tests, respectively, in the presence of Fe2SO4
as the mordant. The antibacterial nature of prodigiosin solutions
and their good textile fastness to fabrics could be essential in manufacturing antiseptic materials such as bandages,
hospital clothing and agricultural applications such as tubers preservation.
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Citation
https://doi.org/10.1186/s13568-023-01579-y