Aqueous and Methanolic Extract of Ocimum Gratissimum (Linn.) Leaf Reversibly Normalizes the Antioxidant Activities of Rats with Gentamicin Induced Liver Injury
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Date
2019-09-03
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Abstract
Objective: the objective of the work is to investigate the
ameliorative effect of aqueous and methanolic extract of ocimum
gratissimum (linn) on gentamicin-induced liver injury. Forty five
adult male Wistar rats were used for this study. They were
divided into 5 groups as follows: Group 1 (the control) (n = 5)
received distilled water daily by oral route for the whole period
of the study. Group 2 (the toxic control) (n = 10) received 100
mg/kg/day of gentamicin i.p. for a week. Groups 3, 4, and 5 (n =
5) were pre-treated with gentamicin as the Group 2 rats, after
which they received 100, 200 and 400 mg/kg/day each of AOGL
p.o., Groups 6, 7, and 8 (n = 5) were pre-treated with gentamicin
as the Group 2 rats, after which they received 100, 200 and 400
mg/kg/day each of MOGL p.o., respectively, for 14 days.
Markers of liver function such as ALT, AST, ALP, Total
bilirubin, conjugated bilirubin and total protein were
determined in the plasma. Oxidative stress markers such as
TBARS and GSH were assayed in the tissue homogenate.
Gentamicin treatment induced significant (p < 0.05) increases in
ALT, AST, ALP, Total bilirubin and total protein and TBARS in
the toxic control when compared to the control group.
Significant decreases (p < 0.05) in GSH was also associated with
gentamicin administration. Post-treatment with AOGL caused
significant increases in and GSH, and significant (p < 0.05)
decreases in ALT, AST, ALP, Total bilirubin and total protein in
the treated groups when compared with the toxic control group.
The results of this study indicated that AOGL ameliorated the
liver injury caused by gentamicin in rats. Hence, the extracts
have the potential of being used for the management of
gentamicin-induced liver injury.