Ascorbic and salicylic acids modulate the binding interactions of an emergency contraceptive pill levonorgestrel to a model transport protein
| dc.contributor.author | Martins O. Omorogie | |
| dc.date.accessioned | 2025-05-07T09:35:13Z | |
| dc.date.available | 2025-05-07T09:35:13Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Serum proteins generally help to transport and distribute drug molecules within the body. In this study, the binding characteristics of bovine serum albumin (BSA) with levonorgestrel (LVG), an emergency contraceptive pill, and the influences of ascorbic acid (ASC) and salicylic acid (SAL) on the binding behaviour and protein structure were elucidated using multi-spectroscopic techniques and molecular docking. The results showed that levonorgestrel decreased BSA intrinsic fluorescence via static quenching mechanism. Binding constant (Ka) values for BSA-LVG complexes were 103 to 104 M-1, indicating their high stabilities. Site probing/docking analysis indicated LVG bound between BSA subdomains IIA and IIIA. UV–visible absorption, Fourier Transform-Infrared and 3D fluorescence spectroscopies affirmed LVG-induced changes in BSA structure, especially in α-helix and β-sheet contents. ASC and SAL influenced BSA conformation for LVG binding and reduced the Ka values by 3.37 and 5.43-folds, respectively. LVG altered the microenvironments of tyrosine residues, interacted with Arg-217, Lys-221, Val-292, Glu-443 etc. within the binding domains. The process was spontaneous (ΔG<0), entropy driven (TΔS>ΔH) and involved van der Waals forces and hydrogen bonding. The findings of the study offered details on the binding interaction between BSA and LVG, and also indicated that prior intake of ASC or SAL could suppress the binding affinity of BSA for levonorgestrel. | |
| dc.identifier.uri | https://doi.org/10.1016/j.molstruc.2023.136835 | |
| dc.identifier.uri | https://repository.run.edu.ng/handle/123456789/4402 | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.relation.ispartofseries | - | |
| dc.title | Ascorbic and salicylic acids modulate the binding interactions of an emergency contraceptive pill levonorgestrel to a model transport protein | |
| dc.title.alternative | Insights from spectroscopy and molecular docking analysis | |
| dc.type | Article |