Anti-biofilm Activity of Indigenous Dentrifices MassulariaAcuminata LinnandDistemonanthusbenthamianus Baill on biofilm forming cariogenic bacteria
| dc.contributor.author | Sossou, Ibukun T. | |
| dc.date.accessioned | 2025-05-21T17:46:48Z | |
| dc.date.available | 2025-05-21T17:46:48Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Biofilms are heterogeneous conglomerate of microorganisms which play a significant role in causing dental caries in human. This study investigated the characteristics and antibiotics susceptibility profile of biofilm forming bacteria associated with dental caries. Questionnaire was administered and dental caried swab samples were collected from patients in two dental centers, in Abeokuta, Ogun state, Nigeria. Biochemical and molecular techniques were used for further identification and characterization of bacterial isolates. Tube assay method was used to screen for biofilm forming bacteria. Sensitivity test was done using agar well-diffusion techniques on commonly used toothpaste, indigenous dentifrices (Massularia acuminata and Distemonanthus benthamianus) and antibiotics. Prevalence of caries was higher among females (59.8%) compared to males (40.2%). The age of patients mostly affected ranged between 20 and35 years. The molar teeth were majorly affected with caries (72.5%), with the left lower jaws mostly infected (46.1%). Out of 274 bacterial isolates, Streptococcus mutans was the most prevalent (22.6%), Staphylococcus aureus (19.7%), Enterobacteraerogenes (15.0%), Klebsiellapneumonia (12.8%), followed by Lactobacillussalivarius (11.3%), Bacillus subtilis (10.7%) and Escherichia coli (8.0%) respectively. Biofilm formation assay showed that out of 274 isolates 197 (71.9%) were biofilm formers. There was a significant difference (p<0.05) between the toothpastes used. There was no significant difference (p>0.05) in inhibitory effect between M. acuminata aqueous compared with D. benthamianus. Gram-positive bacteria were sensitive to erythromycin, while the Gram negative bacteria were sensitive to septrin. This study showed that dental caries in Abeokuta affects all ages and groups with pathogenic Streptococcus mutans been the most frequently occurring cariogens. Toothpastes are more effective than the use of indigenous dentifrices in preventing dental caries. | |
| dc.identifier.other | 3768-3780 | |
| dc.identifier.uri | https://repository.run.edu.ng/handle/123456789/4596 | |
| dc.language.iso | en | |
| dc.publisher | Nigerian Journal of Microbiology | |
| dc.relation.ispartofseries | Vol. 31; No. 1 | |
| dc.title | Anti-biofilm Activity of Indigenous Dentrifices MassulariaAcuminata LinnandDistemonanthusbenthamianus Baill on biofilm forming cariogenic bacteria | |
| dc.type | Article |