Antibiotic Resistant Pattern of Some Pathogenic Bacteria and Candida albicans Isolated from Asymptomatic Adolescents and their Susceptibility to Four Medicinal Plant Extracts.
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Date
2013
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Publisher
International Journal of Advanced Research
Abstract
Sexually transmitted diseases pose severe risks to human health. This study
was designed to investigate the state of cleanliness and hygienic practices of
adolescents using secondary school age females as case study. One hundred
vaginal samples were collected from females within the age range of 9-
20years that attended an urban and a rural general Hospital in Ijebu- North
East Local Government Area of Ogun State, Southwest Nigeria with the aid
of sterile swab sticks. Wellstructured questionnaire was used to obtain vital
information about the students. The samples were processed and identified
according to the standard methods. The plant extracts tested were Garcinia
kola, Cola milleni, Vernoniaamygdalina, and Brideliaferrugineawhile the
antibiotics used were commercial antibiotic disk. Agar disc and well
diffusion methods were employed in determining the effect of antibiotics and
plant extracts respectively on the isolated organisms. Enzymatic activity was
used in determining the pathogenicity of the organisms. Staphylococcus
aureus (42%), Lactobacillus species (24%), Escherichia coli (11%), Proteus
species (7%), Pseudomonas aeruginosa (3%) and Candida albicans (13%)
were isolated. Four typed bacteria were used as control. The age groups 18-
20 and 15-17 had the highest occurrence with a frequency rate of 48% and
27% respectively. The enzymatic profile revealed high enzymatic activity.
Both the antibiotics and the extracts revealed highest inhibitory effects
against the standard organisms than the isolated ones. Pefloxacin exhibited
the highest inhibitory zone of 9.00 mm and 8.00 mm against both the control
and isolated E. coliand P. aeruginosarespectively (P>0.005). Cola milleni
had the highest inhibitory effect of 28 mm against P. vulgaris (P< 0.005).
The study revealed that the studied subjects harboured pathogenic organisms,
also the four plant extracts had more inhibitory effects on the organisms than
conventional drugs used.
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Keywords
Asymptomaticadolescents, Vaginal secretion, Microorganisms, Antibiotics, Plant extracts, Antimicrobial activity