Investigation of the Binding Interaction of α-amylase with Chrysophyllum albidum Seed Extract and its Silver Nanoparticles: A Multi-spectroscopic Approach

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dc.contributor.authorOmorogie, Martins
dc.date.accessioned2022-02-02T15:07:33Z
dc.date.available2022-02-02T15:07:33Z
dc.date.issued2020-10-01
dc.description.abstractThe interactions between α-amylase, one of the key enzymes linked with postprandial glucose regulation, and Chrysophyllum albidum seed methanolic extract(CSME) and its green-synthesized silver nanoparticles (CSAgNP) were investigated using multiple spectroscopy including Fourier Transform-Infrared (FT-IR), ultraviolet (UV)-visible absorption, fluorescence spectroscopy, and biochemical analysis. FT-IR spectroscopy revealed presence of some functional groups in the samples. CSME and CSAgNP inhibited α-amylase. The intrinsic fluorescence intensity of α-amylase was quenched by CSME and CSAgNP via static mechanisms, indicating formation of complex between the enzyme and inhibitors. α-Amylase-CSAgNP complex had higher binding constants. The binding processes were exothermic, entropy driven, spontaneous, and involved hydrogen bonds and van der Waals force. Synchronous fluorescence quenching indicated alteration in microenvironment of α-amylase catalytic site tyrosine residues.FT-IR spectroscopy revealed shifts in amide I peak position of α-amylase due to interaction with CSME/CSAgNP. Absorption spectroscopy also affirmed changes in enzyme conformation. This study may provide theoretical basis for designing novel α-amylase inhibitors.en_US
dc.identifier.citation6en_US
dc.identifier.otherhttps://doi.org/10.1016/j.cdc.2020.100517
dc.identifier.urihttp://dspace.run.edu.ng:8080/jspui/handle/123456789/1004
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectChrysophyllum albidumen_US
dc.subjectSilver Nanoparticlesen_US
dc.subjectα-Amylase inhibitionen_US
dc.subjectProtein-Ligand Binding Interactionen_US
dc.subjectFluorescence Quenchingen_US
dc.subjectSpectroscopyen_US
dc.titleInvestigation of the Binding Interaction of α-amylase with Chrysophyllum albidum Seed Extract and its Silver Nanoparticles: A Multi-spectroscopic Approachen_US
dc.typeArticleen_US
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