Browsing by Author "Sossou, Ibukun T."

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    Antibiofilm Activity of Indigenous Dentrifices Massularia acuminata Linn and Distemonanthus benthamianus Baill on Biofilm Forming Cariogenic Bacteria.
    (Nigerian Journal of Microbiology, 2017) Sossou, Ibukun T.
    Biofilms are heterogeneous conglomerate of microorganisms which play a significant role in causing dental caries in human. This study investigated the characteristics and antibiotics susceptibility profile of biofilm forming bacteria associated with dental caries. Questionnaire was administered and dental caried swab samples were collected from patients in two dental centers, in Abeokuta, Ogun state, Nigeria. Biochemical and molecular techniques were used for further identification and characterization of bacterial isolates. Tube assay method was used to screen for biofilm forming bacteria. Sensitivity test was done using agar well-diffusion techniques on commonly used toothpaste, indigenous dentifrices (Massularia acuminata and Distemonanthus benthamianus) and antibiotics. Prevalence of caries was higher among females (59.8%) compared to males (40.2%). The age of patients mostly affected ranged between 20 and35 years. The molar teeth were majorly affected with caries (72.5%), with the left lower jaws mostly infected (46.1%). Out of 274 bacterial isolates, Streptococcus mutans was the most prevalent (22.6%), Staphylococcus aureus (19.7%), Enterobacteraerogenes (15.0%), Klebsiellapneumonia (12.8%), followed by Lactobacillussalivarius (11.3%), Bacillus subtilis (10.7%) and Escherichia coli (8.0%) respectively. Biofilm formation assay showed that out of 274 isolates 197 (71.9%) were biofilm formers. There was a significant difference (p<0.05) between the toothpastes used. There was no significant difference (p>0.05) in inhibitory effect between M. acuminata aqueous compared with D. benthamianus. Gram-positive bacteria were sensitive to erythromycin, while the Gram negative bacteria were sensitive to septrin. This study showed that dental caries in Abeokuta affects all ages and groups with pathogenic Streptococcus mutans been the most frequently occurring cariogens. Toothpastes are more effective than the use of indigenous dentifrices in preventing dental caries.
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    Antibiotic Resistant Pattern of Some Pathogenic Bacteria and Candida albicans Isolated from Asymptomatic Adolescents and their Susceptibility to Four Medicinal Plant Extracts.
    (International Journal of Advanced Research, 2013) Sossou, Ibukun T.
    Sexually transmitted diseases pose severe risks to human health. This study was designed to investigate the state of cleanliness and hygienic practices of adolescents using secondary school age females as case study. One hundred vaginal samples were collected from females within the age range of 9- 20years that attended an urban and a rural general Hospital in Ijebu- North East Local Government Area of Ogun State, Southwest Nigeria with the aid of sterile swab sticks. Wellstructured questionnaire was used to obtain vital information about the students. The samples were processed and identified according to the standard methods. The plant extracts tested were Garcinia kola, Cola milleni, Vernoniaamygdalina, and Brideliaferrugineawhile the antibiotics used were commercial antibiotic disk. Agar disc and well diffusion methods were employed in determining the effect of antibiotics and plant extracts respectively on the isolated organisms. Enzymatic activity was used in determining the pathogenicity of the organisms. Staphylococcus aureus (42%), Lactobacillus species (24%), Escherichia coli (11%), Proteus species (7%), Pseudomonas aeruginosa (3%) and Candida albicans (13%) were isolated. Four typed bacteria were used as control. The age groups 18- 20 and 15-17 had the highest occurrence with a frequency rate of 48% and 27% respectively. The enzymatic profile revealed high enzymatic activity. Both the antibiotics and the extracts revealed highest inhibitory effects against the standard organisms than the isolated ones. Pefloxacin exhibited the highest inhibitory zone of 9.00 mm and 8.00 mm against both the control and isolated E. coliand P. aeruginosarespectively (P>0.005). Cola milleni had the highest inhibitory effect of 28 mm against P. vulgaris (P< 0.005). The study revealed that the studied subjects harboured pathogenic organisms, also the four plant extracts had more inhibitory effects on the organisms than conventional drugs used.
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    Biotreatment of Abattoir Wastewater Using Selected Microorganisms at Mandate Market Ilorin, Kwara State.
    (FUTO Journal Series, 2021) Sossou, Ibukun T.
    Slaughterhouse wastewater has a complex composition and very harmful to the environment. The abattoir wastewater at mandate market Ilorin, Kwara State was studied for possible pollutants and biotreatment using indigenous microorganisms. Microbial count was done using pour plate techniques and the physicochemical analyses were done using standard method. Total bacteria count was between 1.60x104 - 2.43x107 Cfu/ml while fungi count was 1.63x103 - 1.8x106Cfu/ml. Findings showed that the various water samples were contaminated with E. coli and other enteric bacteria. This result revealed that the temperature, pH and electric conductivity ranged from 27.8 - 28.40oC, 7.07 - 7.19, and 103 - 887μS/cm respectively, Total Dissolved Solid (TDS) and Total Suspended Solid (TSS) were between 1350.20 - 880 and 76.5 - 2266.0 mg/L respectively, the Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD) and Chemically Oxygen Demand (COD) ranged between 4.5 - 0.2, 54.4 - 130 and 700 - 1240 mg/L respectively. The presence of E. coli and Streptococcus faecalis in the abattoir wastewater may be attributable to the high load of animal excreta in the wastewater.. Most of the fungal isolates were also soil-inhabiting microorganisms as well as common spoilage organisms associated with beef industry.
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    Inhibitory Effect of Selected Microorganisms on Aedes and Anopheles Mosquitoes.
    (FUTO Journal Series, 2021) Sossou, Ibukun T.
    The call for malaria control has increased over the last century. Malaria is an infectious disease caused by Plasmodium. The emergence of insecticides resistant strains has therefore necessitated the need to develop alternative control methods. The objective of this study was to evaluate the inhibitory activities of Bacillus thurigensis. Bacillus subtilis and Pseudomonas aeruginosa against Aedes aegypti and Anopheles gamblae larvae. The bacterial strains were obt0ained from streak culture and subcultured before their insecticides activities were tested. All the bacterial strains were identified as mosquito – toxic after the selective bioassays and the LC50recorded for each bacterial strains. These results indicate that the bacteria are effective against mosquito larvae, so they could be used as an alternative bioinsecticides against mosquitoes.