DSpace Angular :: Browsing by Author "Sossou, Ibukun T."

Browsing by Author "Sossou, Ibukun T."

Now showing 1 - 19 of 19

Anti-biofilm Activity of Indigenous Dentrifices MassulariaAcuminata LinnandDistemonanthusbenthamianus Baill on biofilm forming cariogenic bacteria
(Nigerian Journal of Microbiology, 2017) Sossou, Ibukun T.
Biofilms are heterogeneous conglomerate of microorganisms which play a significant role in causing dental caries in human. This study investigated the characteristics and antibiotics susceptibility profile of biofilm forming bacteria associated with dental caries. Questionnaire was administered and dental caried swab samples were collected from patients in two dental centers, in Abeokuta, Ogun state, Nigeria. Biochemical and molecular techniques were used for further identification and characterization of bacterial isolates. Tube assay method was used to screen for biofilm forming bacteria. Sensitivity test was done using agar well-diffusion techniques on commonly used toothpaste, indigenous dentifrices (Massularia acuminata and Distemonanthus benthamianus) and antibiotics. Prevalence of caries was higher among females (59.8%) compared to males (40.2%). The age of patients mostly affected ranged between 20 and35 years. The molar teeth were majorly affected with caries (72.5%), with the left lower jaws mostly infected (46.1%). Out of 274 bacterial isolates, Streptococcus mutans was the most prevalent (22.6%), Staphylococcus aureus (19.7%), Enterobacteraerogenes (15.0%), Klebsiellapneumonia (12.8%), followed by Lactobacillussalivarius (11.3%), Bacillus subtilis (10.7%) and Escherichia coli (8.0%) respectively. Biofilm formation assay showed that out of 274 isolates 197 (71.9%) were biofilm formers. There was a significant difference (p<0.05) between the toothpastes used. There was no significant difference (p>0.05) in inhibitory effect between M. acuminata aqueous compared with D. benthamianus. Gram-positive bacteria were sensitive to erythromycin, while the Gram negative bacteria were sensitive to septrin. This study showed that dental caries in Abeokuta affects all ages and groups with pathogenic Streptococcus mutans been the most frequently occurring cariogens. Toothpastes are more effective than the use of indigenous dentifrices in preventing dental caries.
Antibacterial Effect of Five Selected Nigerian Medicinal Plant Extracts
(FUW Trends in Science & Technology Journal, 2022) Sossou, Ibukun T.
The search for new drugs to combat infectious diseases and emergence of resistant microorganisms stimulated this research. This study was therefore designed to screen and compare five selected medicinal plants extracts used in Nigeria as traditional medicine for their antibacterial effects. These plants include Harungana madagascariensis Lam. Ex Poir and Enantia chlorantha Oliv. barks, Senna alata Linn., Gossypium hirsutum Linn. and Alstonia bonnie De Wild leaves. Agar well diffusion method was carried out to test Ethanol extracts against reference strains of Escherichia coli, Bacillus subtilis, Salmonella typhi, Shigella flexneri, Pseudomonas aeruginosa and Staphylococcus aureus. Tube dilution method was carried out to determine both Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extracts. Data were analysed using ANOVA at p = 0.05. Harungana madagascariensis, E. chlorantha and S. alata crude extracts exhibited broad spectrum antibacterial activity by inhibiting all the tested bacterial species with 24.3±0.3, 25.7±0.3 and 27.7±0.6 (mm) in diameter zones of inhibition respectively. The MIC of the extracts ranged from 5.0 to 20.0 mg/mL while the MBC ranged from 20.0 to 30.0 mg/mL. Enantia chlorantha, exhibited MIC of 5.0mg/ml against S, aureus and P. aeruginosa. Enantia chlorantha and A. bonnie showed MBC of 20mg/ml against P. aeruginosa and E. coli respectively. The five selected plant extracts exhibited antibacterial activities which justify their use for treatment in traditional medicine. They could serve as alternative sources of antibacterial agents against resistant strains of microorganisms.
Antibiofilm Activity of Indigenous Dentrifices Massularia acuminata Linn and Distemonanthus benthamianus Baill on Biofilm Forming Cariogenic Bacteria.
(Nigerian Journal of Microbiology, 2017) Sossou, Ibukun T.
Biofilms are heterogeneous conglomerate of microorganisms which play a significant role in causing dental caries in human. This study investigated the characteristics and antibiotics susceptibility profile of biofilm forming bacteria associated with dental caries. Questionnaire was administered and dental caried swab samples were collected from patients in two dental centers, in Abeokuta, Ogun state, Nigeria. Biochemical and molecular techniques were used for further identification and characterization of bacterial isolates. Tube assay method was used to screen for biofilm forming bacteria. Sensitivity test was done using agar well-diffusion techniques on commonly used toothpaste, indigenous dentifrices (Massularia acuminata and Distemonanthus benthamianus) and antibiotics. Prevalence of caries was higher among females (59.8%) compared to males (40.2%). The age of patients mostly affected ranged between 20 and35 years. The molar teeth were majorly affected with caries (72.5%), with the left lower jaws mostly infected (46.1%). Out of 274 bacterial isolates, Streptococcus mutans was the most prevalent (22.6%), Staphylococcus aureus (19.7%), Enterobacteraerogenes (15.0%), Klebsiellapneumonia (12.8%), followed by Lactobacillussalivarius (11.3%), Bacillus subtilis (10.7%) and Escherichia coli (8.0%) respectively. Biofilm formation assay showed that out of 274 isolates 197 (71.9%) were biofilm formers. There was a significant difference (p<0.05) between the toothpastes used. There was no significant difference (p>0.05) in inhibitory effect between M. acuminata aqueous compared with D. benthamianus. Gram-positive bacteria were sensitive to erythromycin, while the Gram negative bacteria were sensitive to septrin. This study showed that dental caries in Abeokuta affects all ages and groups with pathogenic Streptococcus mutans been the most frequently occurring cariogens. Toothpastes are more effective than the use of indigenous dentifrices in preventing dental caries.
Antibiotic Resistant Pattern of Some Pathogenic Bacteria and Candida albicans Isolated from Asymptomatic Adolescents and their Susceptibility to Four Medicinal Plant Extracts
(INTERNATIONAL JOURNAL OF ADVANCED RESEARCH, 2013) Sossou, Ibukun T.
Sexually transmitted diseases pose severe risks to human health. This study was designed to investigate the state of cleanliness and hygienic practices of adolescents using secondary school age females as case study. One hundred vaginal samples were collected from females within the age range of 9 20years that attended an urban and a rural general Hospital in Ijebu- North East Local Government Area of Ogun State, Southwest Nigeria with the aid of sterile swab sticks. Wellstructured questionnaire was used to obtain vital information about the students. The samples were processed and identified according to the standard methods. The plant extracts tested were Garcinia kola, Cola milleni, Vernoniaamygdalina, and Brideliaferrugineawhile the antibiotics used were commercial antibiotic disk. Agar disc and well diffusion methods were employed in determining the effect of antibiotics and plant extracts respectively on the isolated organisms. Enzymatic activity was used in determining the pathogenicity of the organisms. Staphylococcus aureus (42%), Lactobacillus species (24%), Escherichia coli (11%), Proteus species (7%), Pseudomonas aeruginosa (3%) and Candida albicans (13%) were isolated. Four typed bacteria were used as control. The age groups 18 20 and 15-17 had the highest occurrence with a frequency rate of 48% and 27% respectively. The enzymatic profile revealed high enzymatic activity. Both the antibiotics and the extracts revealed highest inhibitory effects against the standard organisms than the isolated ones. Pefloxacin exhibited the highest inhibitory zone of 9.00 mm and 8.00 mm against both the control and isolated E. coliand P. aeruginosarespectively (P>0.005). Cola milleni had the highest inhibitory effect of 28 mm against P. vulgaris (P< 0.005). The study revealed that the studied subjects harboured pathogenic organisms, also the four plant extracts had more inhibitory effects on the organisms than conventional drugs used.
Antibiotic Resistant Pattern of Some Pathogenic Bacteria and Candida albicans Isolated from Asymptomatic Adolescents and their Susceptibility to Four Medicinal Plant Extracts.
(International Journal of Advanced Research, 2013) Sossou, Ibukun T.
Sexually transmitted diseases pose severe risks to human health. This study was designed to investigate the state of cleanliness and hygienic practices of adolescents using secondary school age females as case study. One hundred vaginal samples were collected from females within the age range of 9- 20years that attended an urban and a rural general Hospital in Ijebu- North East Local Government Area of Ogun State, Southwest Nigeria with the aid of sterile swab sticks. Wellstructured questionnaire was used to obtain vital information about the students. The samples were processed and identified according to the standard methods. The plant extracts tested were Garcinia kola, Cola milleni, Vernoniaamygdalina, and Brideliaferrugineawhile the antibiotics used were commercial antibiotic disk. Agar disc and well diffusion methods were employed in determining the effect of antibiotics and plant extracts respectively on the isolated organisms. Enzymatic activity was used in determining the pathogenicity of the organisms. Staphylococcus aureus (42%), Lactobacillus species (24%), Escherichia coli (11%), Proteus species (7%), Pseudomonas aeruginosa (3%) and Candida albicans (13%) were isolated. Four typed bacteria were used as control. The age groups 18- 20 and 15-17 had the highest occurrence with a frequency rate of 48% and 27% respectively. The enzymatic profile revealed high enzymatic activity. Both the antibiotics and the extracts revealed highest inhibitory effects against the standard organisms than the isolated ones. Pefloxacin exhibited the highest inhibitory zone of 9.00 mm and 8.00 mm against both the control and isolated E. coliand P. aeruginosarespectively (P>0.005). Cola milleni had the highest inhibitory effect of 28 mm against P. vulgaris (P< 0.005). The study revealed that the studied subjects harboured pathogenic organisms, also the four plant extracts had more inhibitory effects on the organisms than conventional drugs used.
Antibiotics Resistant Pattern of Some Pathogenic Bacteria and Candida Albicans Isolated from Asymptomatic Adolescents and their Susceptibility to Four Medicinal Plant Extracts
(International Journal of Advanced Research, 2013) Sossou, Ibukun T.
Sexually transmitted diseases pose severe risks to human health. This study was designed to investigate the state of cleanliness and hygienic practices of adolescents using secondary school age females as case study. One hundred vaginal samples were collected from females within the age range of 9 20years that attended an urban and a rural general Hospital in Ijebu- North East Local Government Area of Ogun State, Southwest Nigeria with the aid of sterile swab sticks. Wellstructured questionnaire was used to obtain vital information about the students. The samples were processed and identified according to the standard methods. The plant extracts tested were Garcinia kola, Cola milleni, Vernonia amygdalina, and Bridelia ferruginea, while the antibiotics used were commercial antibiotic disks. Agar disc and well diffusion methods were employed in determining the effect of antibiotics and plant extracts respectively on the isolated organisms. Enzymatic activity was used in determining the pathogenicity of the organisms. Staphylococcus aureus (42%), Lactobacillus species (24%), Escherichia coli (11%), Proteus species (7%), Pseudomonas aeruginosa (3%) and Candida albicans (13%) were isolated. Four typed bacteria were used as control. The age groups 18 20 and 15-17 had the highest occurrence with a frequency rate of 48% and 27% respectively. The enzymatic profile revealed high enzymatic activity. Both the antibiotics and the extracts revealed highest inhibitory effects against the standard organisms than the isolated ones. Pefloxacin exhibited the highest inhibitory zone of 9.00 mm and 8.00 mm against both the control and isolated E. coli and P. aeruginosa, respectively (P>0.005). Cola milleni had the highest inhibitory effect of 28 mm against P. vulgaris (P< 0.005). The study revealed that the studied subjects harboured pathogenic organisms, also the four plant extracts had more inhibitory effects on the organisms than conventional drugs used.
Aqueous Leaf Extract of Azadirachta indica Protects Against Gentamicin-Induced Kidney Injury via Decreases in Renal Function, Inflammation, and Apoptosis Markers.
(Journal of Medicinal Food, 0223) Sossou, Ibukun T.
The effect of the aqueous extract of Azadirachta indica (AAI) on gentamicin (GEN)-induced kidney injury was investigated. The study involves 20 adult male Wistar rats (housed in four separate plastic cages) such that graded dos ages of AAI were administered to the experimental group for 14 days per oral (PO) before exposure to GEN toxicity (100 mg/kg) for 1 week. At the end of the study, comparisons of some markers of renal functions, antioxidant status, and inflam matory and apoptotic markers were made between the control, GEN, and AAI-pretreated groups at P < .05. The result showed that GEN treatment caused a significant increase (P < .05) in body weight, kidney weight, urea, bilirubin, kidney injury molecule 1 (KIM 1), cystatin C, malondialdehyde (MDA), reduced glutathione (GSH), tumor necrotic factor alpha (TNF-a), interleukin-1 (IL-2), caspase-3, and B-cell lymphoma-2 associated X (BAX) as well as a significant decrease (P < .05) in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx), and B-cell lymphoma (BCL)-2 level. Pre-treatment with graded doses of AAI caused a significant increase in urea, CAT, and GPx as well as a significant decrease (P < .05) in kidney weight, bilirubin, KIM 1, cystatin C, MDA, GSH, SOD, TNF-a, IL-2, caspase-3, BAX, and BCL-2. There was an appreciable difference in the kidney histology of the AAI pre-treated groups compared with the GEN. Hence, the extract has prophylactic potential in managing GEN-induced nephrotoxicity by decreasing the markers of renal function and inflammation and downregulating the markers of apoptosis
Bioactive compounds from fermented Vernonia amygdalina leaf
(In-silico Pharmacology, 2024) Sossou, Ibukun T.
Antibiotic resistance microorganisms (ARMs), particularly gram-negative bacteria, pose a global health threat. The effects of fermentation on phytochemicals are numerous, and exploring this potential is the focus of drug development. The study investigated the role of fermentation in modifying V. amygdalina leaf secondary metabolites as an effective antibiotic against Escherichia. coli, Bacillus subtilis and Salmonella typhi. This work showed that fermentation increased the con tent of lycopene, flavonoid and carotenoid compounds but decreased chlorophyll, soluble protein and phenol. Pearson’s correlation heatmap showed a strong correlation between microbial activities and secondary metabolic changes. The methanolic extract of fermented V. amygdalina leaf pulp (at day 9) showed significant antioxidant and anti-inflammatory activities. The GCMS and FTIR results showed unique compounds and structural modifications at different intervals of the fermentation period. In-vitro and in-silico analyses showed that fermentation did not alter the inhibition rate against B. subtilis; however, E. coli and S. typhi were significantly inhibited by fermented V. amygdalina pulp extracts. In-silico analyses showed that 4,6-Cholestadien-3β-ol– a compound present only on the ninth day of fermentation–was responsible for the inhibition of the gram-negative bacteria via the substitution of multiple non-ionic interactions of some key cata lytic site residues with non-ionic types, thereby denying ionisation and salt-bridge properties that porins explore to resist antibiotics; and higher binding affinity to OmpC and OmpF than ampicillin. Therefore, this steroid-derived compound may open a new pipeline for developing ion-independent multi-target antibiotics against broad-spectrum multidrug-resistant gram-positive and gram-negative bacteria in food and pharmaceutical purposes.
Biotreatment of abattoir waste water using selected microorganisms at Mandate market Ilorin, Kwara state
(Futo Journal Series (FUTOJNLS), 2021) Sossou, Ibukun T.
Slaughterhouse wastewater has a complex composition and very harmful to the environment. The abattoir wastewater at mandate market Ilorin, Kwara State was studied for possible pollutants and biotreatment using indigenous microorganisms. Microbial count was done using pour plate techniques and the physicochemical analyses were done using standard method. Total bacteria count was between 1.60x104 - 2.43x107 Cfu/ml while fungi count was 1.63x103 - 1.8x106Cfu/ml. Findings showed that the various water samples were contaminated with E. coli and other enteric bacteria. This result revealed that the temperature, pH and electric conductivity ranged from 27.8 - 28.40oC, 7.07 - 7.19, and 103 - 887μS/cm respectively, Total Dissolved Solid (TDS) and Total Suspended Solid (TSS) were between 1350.20 - 880 and 76.5 - 2266.0 mg/L respectively, the Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD) and Chemically Oxygen Demand (COD) ranged between 4.5 - 0.2, 54.4 - 130 and 700 - 1240 mg/L respectively. The presence of E. coli and Streptococcus faecalis in the abattoir wastewater may be attributable to the high load of animal excreta in the wastewater.. Most of the fungal isolates were also soil-inhabiting microorganisms as well as common spoilage organisms associated with beef industry.
Biotreatment of Abattoir Wastewater Using Selected Microorganisms at Mandate Market Ilorin, Kwara State.
(FUTO Journal Series, 2021) Sossou, Ibukun T.
Slaughterhouse wastewater has a complex composition and very harmful to the environment. The abattoir wastewater at mandate market Ilorin, Kwara State was studied for possible pollutants and biotreatment using indigenous microorganisms. Microbial count was done using pour plate techniques and the physicochemical analyses were done using standard method. Total bacteria count was between 1.60x104 - 2.43x107 Cfu/ml while fungi count was 1.63x103 - 1.8x106Cfu/ml. Findings showed that the various water samples were contaminated with E. coli and other enteric bacteria. This result revealed that the temperature, pH and electric conductivity ranged from 27.8 - 28.40oC, 7.07 - 7.19, and 103 - 887μS/cm respectively, Total Dissolved Solid (TDS) and Total Suspended Solid (TSS) were between 1350.20 - 880 and 76.5 - 2266.0 mg/L respectively, the Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD) and Chemically Oxygen Demand (COD) ranged between 4.5 - 0.2, 54.4 - 130 and 700 - 1240 mg/L respectively. The presence of E. coli and Streptococcus faecalis in the abattoir wastewater may be attributable to the high load of animal excreta in the wastewater.. Most of the fungal isolates were also soil-inhabiting microorganisms as well as common spoilage organisms associated with beef industry.
Dental Caries Risk Factors Among Symptomatic and Asymptomatic Selected People.
(Biomedicine and Nursing, 2021) Sossou, Ibukun T.
Background: Dental caries, the most common chronic infectious disease that affects humans, is one of the most common preventable diseases. It is now recognized as a major public health oral disease which hinders the achievement and maintenance of oral health in all age groups, sex and tribes all over the world by gradually destroying the tooth structure and causing discomfort. To know the important factors responsible for the formation of tooth decay and use these factors as a guide for prevention. Materials and methods: Useful information was obtained from the study subjects by imploring them to fill questionnaire through the Google Forms Application. The responses received from respondents were analyzed using the Social Sciences Statistical Package (Version 23). Results: Out of the 100 respondents (53 females and 47 males), 68% had tooth decay. Patients between the ages of 21 and 35 years had the highest frequency (57%) out of those who participated in the study. There is a correlation (-0.04) between the dwelling area of the patients and tooth decay. It was observed that the majority (89%) of respondents did not visit the dentist for regular annual oral examination. Conclusion: Due to limited information on risk factors of dental caries in vulnerable populations and a lack of access to dental caries, preventable efforts are hindered. Thus, the need to educate this population on the common risk factors of tooth decay and that early diagnosis should be treated with immediate effect.
Gas Chromatography-Mass Spectroscopy and Antibacterial Activity of Stem Bark of Terminalia Glaucascens on Some Multidrug-Resistant Gram-Negative Bacteria.
(Scientia Africana, 2023) Sossou, Ibukun T.
Frequent antimicrobial resistance of bacterial pathogens has led to a search for plant origin to synthesize new drugs. Hence, the antibacterial potential of the stem bark part of Terminalia glaucescens plant needs to ascertain. The 162 pure bacteria isolated from the patient’s clinical samples were confirmed using standard procedures to be Escherichia coli (65), Pseudomonas aeruginosa (52), and Klebsiella pneumonia (45). The isolates were tested against seven commonly available antibiotics and the extracts. The identified bark of T. glaucescens was prepared for qualitative analysis using standard methods and also analyzed quantitatively using Gas Chromatography-Mass Spectroscopy (GC-MS). Alkaloid, saponin, flavonoid, steroids, tannin, terpenoid, and phenol were identified. The GC-MS analysis revealed 13 bioactive constituents with Neophytadiene, Squalene, 3, 7, 11, 15-Tetramethyl-2-hexadecen-1-ol and 9-eicosyne as active components. Antibiogram study revealed that most isolates, especially E. coli and P. aeruginosa, were 100% resistant to more than two antibiotics. The antibacterial activity of the stem bark of T. glaucescens was reflected by inhibition zones, ranging from 5.5 mm to 12.5 mm; Escherichia coli had the highest zone of inhibition (12.5 mm). The presence of phytochemicals and bioactive components in this stem bark suggests its effectiveness in treating infections and producing pharmaceutical products.
Immune boosting and ameliorative properties of aqueous extract of Vernonia amygdalina Delile against MSG-induced genotoxicity: An in-silico and in-vivo approach.
(Heliyon, 0210) Sossou, Ibukun T.
Vernonia amygdalina (VA) is popularly consumed as food and as medicine due to its nutritional and bioactive constituents. This study assessed the anti-genotoxic effect of aqueous leaf extract of VA against monosodium (MSG) –induced genotoxicity. Crude extraction and phytochemical analysis were done using standard methods. In silico studies was done using compounds in the extract against Bcl-2, NF-kB 50, DNA polymerase lambda, DNA ligase, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Twelve rats were divided into three groups with four rats in each group. Group I was fed on food and water, group II received MSG (4 g/kg) per body weight (pbw) intraperitoneally, group III received MSG (4 g/kg) pbw intraperi toneally followed by oral dose of VA leaf extract (250 mg/kg) per body weight. The number of the micronucleated red blood cells and white blood cells were determined from blood smears microscopically. Results showed that aqueous extract of VA contained in mg/100 g alkaloids (7.04 ± 0.16), saponins (3.91 ± 0.13), flavonoid (1.64 ± 0.16), phenol (3.40 ± 0.12) and tannins (0.07 ± 0.32). In silico studies revealed high binding interaction (ΔG > 8.6) of vernoniosides D and E with all the tested proteins. There was a reduction in the number of micronucleated cells, neutrophils and eosinophils of the treated group compared to the MSG group, while there was an increase in the lymphocyte count. The anti-genotoxic effects of VA leaf extract might be attributed to the synergistic interaction of the various bioactive components in the extract. VA could be a potential plant for the prevention of cancer and other diseases that attenuate the immune system.
In-vitro and In-silico Anti-cariogenic Bacteria Activity of Selected Spices Commonly Consumed in South-West Nigeria.
(Nigerian Journal of Biochemistry and Molecular Biology, 2024) Sossou, Ibukun T.
Dental caries is a preventable oral disease simply by practicing good oral hygiene; however, this disease remains a global health challenge due to the difficulties in management and treatment. Onion, ginger, and nutmeg extracts have been reported as potent antibiotics. Thus, the study investigated the efficacy of these three spices against cariogenic bacteria. Samples were collected from consented caried-patients at the Olabisi Onabanjo University Teaching Hospital (OOUTH), Sagamu, Ogun State, Nigeria. Disc and agar well diffusion methods were used to evaluate the antibiotic sensitivity of the spices against the isolates. The anti-cariogenic potential of significant compounds in each of the spices was further assessed against four bacterial target proteins (DNA gyrase B, dihydrofolate reductase (DHF), D-alanine: D-alanine ligase (Ddl1), and Isoleucyl-tRNA synthetase (IARS). Out of 100 samples collected, hundred and sixty-six (166) isolates were recovered: Pseudomonas aeruginosa (105), Staphylococcus aureus (52), and Streptococcus mutans (9). In-silico studies revealed that kaempferol in onion, luteolin 7-O-glucoside in ginger, and macelignan in nutmeg had high binding affinities for the four target proteins (∆G> -7 kcal/mol). At 100% (1 g/mL) concentration, onions showed a higher zone of inhibition (20-22 mm) against the tested bacteria. In comparison, diameter zones of 20.33 mm and 20.67 mm for ginger, and 19.67 mm and 16.67 mm for nutmeg were observed against Streptococcus mutans and Pseudomonas aeruginosa, respectively. It was also observed that at higher concentrations of 100%, the tested spices had a higher inhibitory effect against the tested bacteria than the control antibiotics.
Inhibitory effect of selected microorganisms on Aedes and Anopheles mosquitoes
(Futo Journal Series (FUTOJNLS), 2021) Sossou, Ibukun T.
The call for malaria control has increased over the last century. Malaria is an infectious disease caused by Plasmodium. The emergence of insecticides resistant strains has therefore necessitated the need to develop alternative control methods. The objective of this study was to evaluate the inhibitory activities of Bacillus thurigensis. Bacillus subtilis and Pseudomonas aeruginosa against Aedes aegypti and Anopheles gamblae larvae. The bacterial strains were obt0ained from streak culture and subcultured before their insecticides activities were tested. All the bacterial strains were identified as mosquito – toxic after the selective bioassays and the LC50recorded for each bacterial strains. These results indicate that the bacteria are effective against mosquito larvae, so they could be used as an alternative bioinsecticides against mosquitoes.
Inhibitory Effect of Selected Microorganisms on Aedes and Anopheles Mosquitoes.
(FUTO Journal Series, 2021) Sossou, Ibukun T.
The call for malaria control has increased over the last century. Malaria is an infectious disease caused by Plasmodium. The emergence of insecticides resistant strains has therefore necessitated the need to develop alternative control methods. The objective of this study was to evaluate the inhibitory activities of Bacillus thurigensis. Bacillus subtilis and Pseudomonas aeruginosa against Aedes aegypti and Anopheles gamblae larvae. The bacterial strains were obt0ained from streak culture and subcultured before their insecticides activities were tested. All the bacterial strains were identified as mosquito – toxic after the selective bioassays and the LC50recorded for each bacterial strains. These results indicate that the bacteria are effective against mosquito larvae, so they could be used as an alternative bioinsecticides against mosquitoes.
International Journal of Advanced Research
(RESEARCH ARTICLE INTERNATIONAL JOURNAL OF ADVANCED RESEARCH, 2013) Sossou, Ibukun T.
Sexually transmitted diseases pose severe risks to human health. This study was designed to investigate the state of cleanliness and hygienic practices of adolescents using secondary school age females as case study. One hundred vaginal samples were collected from females within the age range of 9 20years that attended an urban and a rural general Hospital in Ijebu- North East Local Government Area of Ogun State, Southwest Nigeria with the aid of sterile swab sticks. Wellstructured questionnaire was used to obtain vital information about the students. The samples were processed and identified according to the standard methods. The plant extracts tested were Garcinia kola, Cola milleni, Vernoniaamygdalina, and Brideliaferrugineawhile the antibiotics used were commercial antibiotic disk. Agar disc and well diffusion methods were employed in determining the effect of antibiotics and plant extracts respectively on the isolated organisms. Enzymatic activity was used in determining the pathogenicity of the organisms. Staphylococcus aureus (42%), Lactobacillus species (24%), Escherichia coli (11%), Proteus species (7%), Pseudomonas aeruginosa (3%) and Candida albicans (13%) were isolated. Four typed bacteria were used as control. The age groups 18 20 and 15-17 had the highest occurrence with a frequency rate of 48% and 27% respectively. The enzymatic profile revealed high enzymatic activity. Both the antibiotics and the extracts revealed highest inhibitory effects against the standard organisms than the isolated ones. Pefloxacin exhibited the highest inhibitory zone of 9.00 mm and 8.00 mm against both the control and isolated E. coliand P. aeruginosarespectively (P>0.005). Cola milleni had the highest inhibitory effect of 28 mm against P. vulgaris (P< 0.005). The study revealed that the studied subjects harboured pathogenic organisms, also the four plant extracts had more inhibitory effects on the organisms than conventional drugs used.
Phytochemical and Antioxidant Analysis of Artocarpus heterophyllus Peel Extracts.
(ASFI Research Journal, 2024) Sossou, Ibukun T.
BACKGROUND: Plants have a diverse variety of bioactive compounds such as tannins, alkaloids, flavonoids, carbohydrates and steroids among many others that can offer significant physiological actions in the human body. Phytochemicals are naturally occurring chemical, biological and active compounds found in plants that are of benefit to human health apart from those that act as macronutrients and micronutrients. A. heterophyllus (Jackfruit) belongs to the family Moraceae. Jackfruit tree is native to India and is popular in several tropical and sub-tropical Countries. OBJECTIVE: The aim of this study is to determine the phytochemical constituents, antioxidant properties and gas chromatography mass spectroscopy (GC-MS) of the fruit peel of A. heterophyllus extracts using three different solvents. METHODS: Artocarpus heterophyllus fruits were collected from University of Ibadan botanical garden, identified at the Forestry Research Institute of Nigeria, Oyo State. The peel was extracted and dried at 35 ℃. Qualitative method and Gas Chromatography Mass Spectroscopy were used to determine the phytochemical and bioactive compounds present in the plant, whereas 2, 2, Diphenylpicryl-1 hydrazyl (DPPH) was used for the antioxidant analysis. RESULTS: Secondary metabolites such as flavonoids, phenols, alkaloids, glycosides, saponins, tannins, steroids, and Terpenoids were present based on the solvents while anthraquinnes were absent. Flavonoid and phenol were abundant in the three extracts while saponin was moderate. Eight chemical compounds were observed in the plant extracts. Major bioactive components with highest and lowest retention peak were (65.55 %) bis(2- ethyl hexyl) phthalate and (6.06 %) Cis- 13(2- ethylhexyl)phthalate respectively. Antioxidant activity increased with increase in concentration of which acetone extract had the highest DPPH (75.00 %) at 100 𝜇g/ml. CONCLUSION: The fundamental means of the usage of medicinal plants for medicine is due to their bioactive properties which are used as substrates for biochemical and enzymatic reactions. Phytochemical derivatives have antiviral, anti-inflammatory, anti-oxidant, immunomodulatory, anticancer, antimicrobial, antitumor, analgesic and many other properties harbor in them.
Phytochemical Screening and Antibacterial Activity of Two Plant Extracts against Multidrug-Resistant Stenotrophomonas maltophilia of Clinical Origin.
(Nigerian Journal of Microbiology, 2023) Sossou, Ibukun T.
New antibacterial agents are pressingly in need to combat the outgrowing incidence of bacterial resistance, which has been number one significant public health threat. The antibacterial activity and phytochemical components of Cannabis sativa and Garcinia kola against Stenotrophomonas maltophilia from diabetic patients with wound infection were investigated. Pure and type isolates of S. maltophilia (ATCC 17666) were obtained from Ogun and Lagos State hospitals, while the plants used were purchased from Ago-Iwoye market, Ogun State. Disk and agar diffusion methods were used to evaluate the antibacterial profile, and the effect of the extracts on the studied isolates, respectively. The isolates of S. maltophilia were resistant to all the antibiotics tested. The effect of the two extracts on the two S. maltophilia varied based on the plant solvent, concentration, and organisms. Ethanol extracts of G. kola and C. sativa on pure/type S. maltophilia had the highest diameter zones of inhibition of 24 mm/27 mm and 19 mm/22 mm at 150 mg/ml, respectively. The type isolate had the highest diameter zones of inhibition in all concentrations of the solvent extracts except for the aqueous. Minimum inhibitory and bactericidal concentration for both plants were 75 mg/ml (type isolate), 150 mg/ml (pure isolate), and 75 mg/ml for C. Sativa alone. Some phytochemicals such as alkaloids, flavonoids, tannins, saponins were observed in the plant extracts.In conclusion, the tested plants possess antibacterial activity, hence, could be used as substitutes in the treatment and management of S. maltophilia pathogens in diabetics with wound infection, and also, in the formulation of pharmaceuticals.

Browsing by Author "Sossou, Ibukun T."

Results Per Page

Sort Options