Department of Biochemistry

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Acid Activation of Groundnut Husk for Copper Adsorption: Kinetics and Equilibrium Studies
(Desalination Publications., 2017-08) Olukanni, Olumide
In this study, the use of cheap unmodified and acid-modified agricultural waste primed from groundnut husk as adsorbents for the removal of Cu(II) ions from aqueous solution were examined in a batch process under different experimental conditions. Fourier transform infrared characterization of the adsorbent before and after the adsorption process revealed the likely involvement of various functional groups such as –COOH, –C=O, –NH2 and –OH− groups. Structural and morphological changes of the biomass were equally observed by X-ray diffraction and scanning electron microscopy analysis before and after adsorption of copper ions. Optimum experimental conditions were obtained to be the initial metal concentration of 50 mg L−1, contact time of 70 min, biomass dosage of 0.03 g and hydrogen ion concentration of 5.0. The kinetic studies showed that the sorption pattern of both biomasses followed pseudo-second-order model, while the isotherms studies revealed that the adsorption data were well governed by Freundlich adsorption isotherm. The monolayer adsorption capacity for unmodified groundnut husk was found to be 14.525 and 20.146 mg g−1 for acid-modified groundnut husk as computed from the Langmuir model equation. It can thus be concluded that waste groundnut husk can be used as a low cost and environmentally friendly adsorbent for Cu(II) ions removal from aqueous solution.
Acid Activation of Groundnut Husk for Copper Adsorption: Kinetics and Equilibrium Studies
(Desalination Publications, 2017-08) Olukanni, Olumide
In this study, the use of cheap unmodified and acid-modified agricultural waste primed from groundnut husk as adsorbents for the removal of Cu(II) ions from aqueous solution were examined in a batch process under different experimental conditions. Fourier transform infrared characterization of the adsorbent before and after the adsorption process revealed the likely involvement of various functional groups such as –COOH, –C=O, –NH2 and –OH− groups. Structural and morphological changes of the biomass were equally observed by X-ray diffraction and scanning electron microscopy analysis before and after adsorption of copper ions. Optimum experimental conditions were obtained to be the initial metal concentration of 50 mg L−1, contact time of 70 min, biomass dosage of 0.03 g and hydrogen ion concentration of 5.0. The kinetic studies showed that the sorption pattern of both biomasses followed pseudo-second-order model, while the isotherms studies revealed that the adsorption data were well governed by Freundlich adsorption isotherm. The monolayer adsorption capacity for unmodified groundnut husk was found to be 14.525 and 20.146 mg g−1 for acid-modified groundnut husk as computed from the Langmuir model equation. It can thus be concluded that waste groundnut husk can be used as a low cost and environmentally friendly adsorbent for Cu(II) ions removal from aqueous solution.
Aerobic Bacterial Degraders in Effluent from Itoku Textile Industry, Abeokuta
(Academic Journals: African Journal of Biotechnology, 2013-12-11) Olukanni, Olumide
The local textile industry in Itoku village is one major textile industry in Abeokuta Ogun state, known for “adire” production whose processes are not maintained at regulatory standards. This study involves isolating and identifying aerobic microorganisms in waste water effluents from this textile Industry and screening for aerobic degraders among the isolates. Fifty milliliter (50 ml) of three dye effluents; purple, brown and green were collected into sterile bottles and designated (Ef1), (Ef2) and (Ef3). Tap water was used as control (Cont). Isolation, identification and screening of isolates for percentage decolorization were done using standard method. Colony-forming units showed the trend Ef3 > Ef2> Ef1. Bacterial isolates included Bacillus megaterium, Staphylococcus simulans, Micrococcus varians, Bacillus niacinni, Lysinibacillus sp. and Bacillus carbonificus. Aneurinbacillus aneurinilytus alone was isolated from the control sample. Bacillus megaterium and Lysinibacillus sp. were the most predominant species in the effluents. S. simulans was the most efficient degrader and its use could be exploited.
Antimicrobial Activity of Ficus Leaf Extracts on some Fungal and Bacterial Pathogens of Discorea rotundata from Southwest Nigeria
(Journal of Biological Sciences,, 2011) Olukanni, Olumide
Bio-control methods have become a common practice in horticulture and crop husbandry due to the attendant negative impact of fungicides and other chemical agents on soil, vegetation and environment. Most fungicides accumulate in plant tissues and subsequently trigger carcinogenic effects in animals and humans. The leaf extracts of Ficus thonningii, F. saussureana, F. exasperata and F. sur were screened for antimicrobial properties on eight (8) fungal species which included Aspergillus flavus, A. niger, Botryodiploidia theobromae, Fusarium oxysporum, F. solani, Penicillium chrysogenum, P. oxalicum and Rhizopus stolonifer and two (2) bacterial species viz; Pseudomonas spp. and Klebsiella spp. which were isolated from the rot portions of tubers of Dioscorea rotundata. The extracts from the Ficus species had low antimicrobial effect at 25 and 50 mg mL-1 concentrations while a significant arrest of mycelia growth was observed at 75 and 100 mg mL-1 concentrations. The presence of alkaloids, flavonoids and cardiac glycosides in the leaves of these species may have conferred the antimicrobial properties on these species. Application of the fungal pathogens isolated on healthy tubers and the subsequent development of rots confirmed these organisms as the natural pathogens of this crop. The extracts from all the four Ficus species exerted significant antimicrobial effect on all the test organisms at 75 and 100 mg mL-1 concentrations and its application at these concentrations would help to minimise infection and spoilage during and after storage and improve farmers’ revenue.
Antimutagenic Activity of Seeds of Moringa Oleifera,
(CRC Press (Taylor and Francis group), 2021) Olukanni, Olumide
Moringa oleifera has medicinal and nutritional values. This study was aimed at assessing the antimutagenic potential of M. Oleifera seeds. Ethanol was used for the extraction. Chemical analysis was done following standard methods. Antimutagenicity was determined against nitrate treated 1-amino- pyrene in acid solution using the Salmonella assay. Preliminary phytochemical screening of seed extract M. Oleifera revealed tannin, alkaloid, flavonoid, terpenoid, saponin, cardiac glycoside, anthraquinone, and steroid; phlobatanin was not detected. Tannin was found to be in the highest amount (28.2± 0.63) mg/100g, flavonoid (23.21± 0.44) mg/100g, steroid (20.50± 2.15) mg/100g, alkaloid (18.68 ± 2.15) mg/100g and saponin (15.22 ± 0.63) mg/100g. The extract showed a strong percentage inhibition in both strains at higher doses. The observed antimutagenic effect of the seed extract could be adduced to some phytochemical constituents therein.
Biodegradation of Malachite Green by Extracellular Laccase Producing Bacillus thuringiensis RUN1.
(Journal of Basic and Applied Sciences,, 2013) Olukanni, Olumide
Abstract: A bacteria strain Bacillus thuringiensis RUN1 identified by 16s RNA gene phylogenetic analysis was used to decolorize malachite green, a triphenylmethane dye in a simulated wastewater. The ability of the organism to produce extracellular laccase and degrade the dye were also investigated. Results showed that the organism decolorized (84.67± 1.19 %) malachite green at 40 mgl-1 within 6 h; and the decolorization was associated to laccase production by the organism experimentally. Laccase activity increased as the decolorization process progressed, with the highest activity value of 0.1043 ± 0.02 U/min/ mg protein recorded after 24 h of incubation using ABTS as substrate. In addition, FTIR analysis showed that the strain actually degraded the dye. It was therefore concluded that this strain of Bacillus thuringiensis would be relevant in the biotreatment of industrial effluent containing malachite green and in the production of laccase, an industrially important enzyme.
Biosynthesis of Silver Nanoparticles Using Almond Plantleaf Extract and their Antibacterial Activity
(IJESC, 2018-11) Olukanni, Olumide
This work reports the synthesis of silver nanoparticles (AgNPs) using almond leaf extract and the evaluation of its antibacterial activity. Antibacterial activity of the biosynthesized AgNPs was evaluated against some selected pathogenic microorganisms. The biosynthesised AgNPs were characterised using UV-Visible, Fourier Transmission Infrared Spectroscope (FTIR), Energy Dispersive X-ray Spectroscope (EDX) and Field Emission Scanning Electron Microscope (FESEM). The absorption spectrum of the synthesized AgNPs showed a maximum spectrum of 430 nm while FTIR analysis showed different functional groups present on the surface of the AgNPs with broad peak between 3000 and 3800 cm−1. The FESEM showed a large number of spherically shaped nanoparticles with sizes ranging from 8.34 to 78.96 nm. The almond leaf extract possess biomolecules which aided the bioreduction, formation and stabilization of the AgNPs, which in turn inhibited distinctly the growth of the selected microorganisms with zones ranging from 9-10 mm. This study showed that almond leaf can be used for the synthesis of AgNPs possessing antibacterial properties.
Chemistry and Industry. In: Science, Technology and Humankind
(The Directorate of General Studies, 2014) Olukanni, Olumide
Natural resources are useful substances that occur within our environment without any known human efforts. The soil, the rocks, the water, the light, crude oil, coal and other mineral resources are all natural resources. These resources could be renewable or non-renewable.
Decolorization and Biodegradation of Reactive Blue 13 by Proteus mirabilis LAG
(Elsevier B.V., 2010) Olukanni, Olumide
The decolorization and biodegradation of Reactive Blue 13 (RB13), a sulphonated reactive azo dye, was achieved under static anoxic condition with a bacterial strain identified as Proteus mirabilis LAG, which was isolated from a municipal dump site soil near Lagos, Nigeria. This strain decolorized RB13 (100 mg/l) within 5 h. The formation of aromatic amine prior to mineralization was supported by Fourier transform infrared spectrometry (FTIR), which revealed the disappearance of certain peaks, particularly those of the aromatic C–H bending at 600–800cm−1. Gas chromatography–mass spectrophotometry (GCMS) analysis of the dye metabolite showed the presence of sodium-2(2-formyl-2-hydroxyvinyl) benzoate, with a tropylium cation as its base peak. This suggested the breakage of naphthalene rings in RB13. The detection of azoreductase and laccase activities suggested the enzymatic reduction of azo bonds prior to mineralization. In addition, phytotoxicity studies indicated the detoxification of RB13 to non-toxic degradation products by this strain of P. mirabilis LAG.
Decolorization Kinetics of Reactive Blue 13 Azo Dye by Proteus mirabilis
(CSIR-NEERI, 2014-10) Olukanni, Olumide
A dye decolorizing bacterium, Proteus mirabilis, isolated from soil was used in batch experiments for decolorization of the sulphonated dye, Reactive blue 13. Effects of initial substrate (dye) and bacterial cell mass concentrations on the specific decolorization rate and extent of dye decolorization were investigated. Aeration as against agitation increased the decolorization rate by 4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye concentration 382.98 mg/L. The decolorization rate by Proteus mirabilis was found to follow half (0.5498) and first (1.0977) order kinetics with respect to dye concentration and cell mass concentrations respectively. The specific decolorization rate estimated from the experimental data was 37.03 mg/L/h/g dry cell mass (DCM), and the value of the apparent Km was 284.76 mg/L. This study showed that the strain of Proteus mirabilis has potential in wastewater color removal.
Decolorization of Azo Dyes by a Strain of Micrococcus Isolated from a Refuse Dump Soil.
(Biotechnology, 2009) Olukanni, Olumide
Bacterial degradation is a viable treatment option for azo dyes containing wastewater. However, a great drawback is the generation of potentially toxic and mutagenic end products (aromatic amines) by anaerobic bacteria. This study is part of efforts to develop textile effluent bio-treatment processes to produce reusable water by decolourization and degradation of azo dyes to non-toxic metabolites. The ability of fourteen bacterial strains isolated from various environmental sources to decolourize textile wastewaters aerobically using a simulated effluent made with three textile reactive azo dyes; Reactive Yellow 42 (RY 42), Reactive Blue 13 (RB 13) and Reactive Red 58 (RR 58) were investigated. Three strains showed >95% decolourization of the synthetic effluent within 24 h. The effect of culture condition (pH, temperature and media) on the degradation of methyl red, a standard azo dye, by the isolate with the highest decolourization level; identified as Micrococcus sp., was also studied. The strain showed optimum decolourization at pH and temperature around 7 and 37°C, respectively. It preferred nutrient broth to minimal media and 0.02 g dry mass decolourized 50 mL, 56 mg L-1 solution of methyl red within 6 h under adequate oxygen supply. UV-visible spectra analyses of aniline sulphate (an aromatic amine) and those of the metabolic products of methyl red suggest that methyl red was first converted to aromatic amine(s) which was subsequently mineralized by the bacterium. The high azo dyes' decolourization ability of the Micrococcus strain suggested that aerobic decolourization of azo dyes could be as effective as the anaerobic counterpart if suitable organisms are employed.
Decolorization of Dye House Effluent and Biodegradation of Congo red by Bacillus thurengesis RUN1
(Journal of Microbiology and Biotechnology,, 2013) Olukanni, Olumide
A dye-decolorizing bacterium was isolated from a soil sample and identified as Bacillus thuringiensis using 16S rRNA sequencing. The bacterium was able to decolorize three different textile dyes, namely, Reactive Blue 13, Reactive red 58, Reactive yellow 42, and a real dyehouse effluent up to 80-95% within 6 h. Some non-textile industrially important dyes were also decolorized to different extents. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometer analysis of the ethyl acetate extract of Congo red dye and its metabolites showed that the bacterium could degrade it by the asymmetric cleavage of the azo bonds to yield sodium (4- amino-3-diazenylnaphthalene-1-sulfonate) and phenylbenzene. Sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) was further oxidized by the ortho-cleavage pathway to yield 2- (1-amino-2-diazenyl-2-formylvinyl) benzoic acid. There was the induction of the activities of laccase and azoreductase during the decolorization of Congo red, which suggests their probable role in biodegradation. B. thuringiensis was found to be versatile and could be used for industrial effluent biodegradation.
Decolorizing Kinetics of Reactive Blue 13 Azo Dye by Proteus mirabilis
(NEERI J Environ Sci Engg, 2014-10) Olukanni, Olumide
A dye decolorizing bacterium, Proteus mirabilis, isolated from soil was used in batch experiments for decolorization of the sulphonated dye, Reactive blue 13. Effects of initial substrate (dye) and bacterial cell mass concentrations on the specific decolorization rate and extent of dye decolorization were investigated. Aeration as against agitation increased the decolorization rate by 4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye concentration 382.98 mg/L. The decolorization rate by Proteus mirabilis was found to follow half (0.5498) and first (1.0977) order kinetics with respect to dye concentration and cell mass concentrations respectively. The specific decolorization rate estimated from the experimental data was 37.03 mg/L/h/g dry cell mass (DCM), and the value of the apparent Km was 284.76 mg/L. This study showed that the strain of Proteus mirabilis has potential in wastewater color removal.
Degradation of Indigo Dye by a Newly Isolated Yeast, Diutina rugosa from Dye Wastewater Polluted Soil
(Elsevier, Journal of Environmental Chemical Engineering, 2017-09-02) Olukanni, Olumide
Isolation, identification, and characterization of newly isolated yeast, Diutina rugosa capable of decolorizing indigo dye were investigated in this study. Molecular and phylogenetic analyses of 23S rRNA sequence data indicated that the yeast belonged to the new genus, Diutina. The optimization of physicochemical parameters such as pH of the solution (2–8), initial dye concentration (10–60 mg L−1), adsorbent mass (0.1–2 g), and temperature (10–50 °C) was studied to scale- up the conditions of dye removal. Furthermore, complete decolorization (99.97%) of indigo dye (10 mg L−1) was achieved at pH 2, temperature 30 °C and 2.0 g cell biomass within 5 days. Degradation was monitored through UV–vis spectrophotometric, FTIR, and GCMS analyses. The results of FTIR analysis obtained revealed the loss and shifts in spectra peaks of the experimental in comparison with the biological control. Possible degradation pathway was proposed using the intermediate metabolites; 1, 2-dihydro-3H-indol-3-one and cyclopentanone obtained through GCMS analysis. The enzyme analyses revealed significant inductions and major roles played by NADH-DCIP reductase and lignin peroxidase in the asymmetric cleavage, initial reduction and deamination of indigo dye. The equilibrium experimental data were fitted to Langmuir, Freundlich and Temkin adsorption isotherms with high adjusted coefficient of determination values; adjR2 =0.907, adjR2 =0.867, and adjR2 =0.965 respectively. However, the Langmuir and Temkin adsorption isotherms affirmed the monolayer and heterogeneous biosorption characteristics of Diutina rugosa. Temkin adsorption isotherm model (R2 =0.971) represented the best fit of experimental data than other isotherm models.
Degradation of Indigo Dye by a Newly Isolated Yeast, Diutina Rugosa from Dye Wastewater Polluted Soil
(Elsevier, 2017-09) Olukanni, Olumide
Isolation, identification, and characterization of newly isolated yeast, Diutina rugosa capable of decolorizing indigo dye were investigated in this study. Molecular and phylogenetic analyses of 23S rRNA sequence data indicated that the yeast belonged to the new genus, Diutina. The optimization of physicochemical parameters such as pH of the solution (2–8), initial dye concentration (10–60 mg L−1), adsorbent mass (0.1–2 g), and temperature (10–50 °C) was studied to scale- up the conditions of dye removal. Furthermore, complete decolorization (99.97%) of indigo dye (10 mg L−1) was achieved at pH 2, temperature 30 °C and 2.0 g cell biomass within 5 days. Degradation was monitored through UV–vis spectrophotometric, FTIR, and GCMS analyses. The results of FTIR analysis obtained revealed the loss and shifts in spectra peaks of the experimental in comparison with the biological control. Possible degradation pathway was proposed using the intermediate metabolites; 1, 2-dihydro-3H-indol-3-one and cyclopentanone obtained through GCMS analysis. The enzyme analyses revealed significant inductions and major roles played by NADH-DCIP reductase and lignin peroxidase in the asymmetric cleavage, initial reduction and deamination of indigo dye. The equilibrium experimental data were fitted to Langmuir, Freundlich and Temkin adsorption Isotherms with high adjusted coefficient of determination values; adjR2 =0.907, adjR2 =0.867, and adjR2 =0.965 respectively. However, the Langmuir and Temkin adsorption isotherms affirmed the monolayer and heterogeneous biosorption characteristics of Diutina rugosa. Temkin adsorption isotherm model (R2 =0.971) represented the best fit of experimental data than other isotherm models.
Ecofriendly Production of Silver Nanoparticles from the Seeds of Carica papaya and Its Larvicidal and Antibacterial Efficacy against Some Selected Bacterial Pathogens
(IOP Conference Series: Materials Science and Engineering, 2020) Olukanni, Olumide
Carica papaya seed extract (CPE) was used in the synthesis of silver nanoparticles (AgNPs) in this study. For the characterisation of biosynthesized particles, UV-vis spectroscopy, FTIR, FESEM and EDX were used. Antimicrobial and larvicidal efficacies of the synthesized AgNPs were assessed in the fight against certain pathogens and the Aedes aegypti 4th instar larvae. The absorption spectrum of AgNPs peaked at 433 nm with a broad peak of 3000 to 3800 cm-1 for different functional groups as presented in the FTIR analysis. A FESEM evaluation revealed a number of spherical particle structures with an average of 20-69 nm. With zones of inhibition between 10-24 mm, the AgNPs synthesized inhibited selected microorganisms. After 12 h of exposure, the nanoparticles had LC50 and LC90 lethal concentrations on the Aedes aegypti larva at 14.56 and 33.89 μg/ml respectively. This study demonstrates the possibility of using Carica papaya seeds in AgNPs synthesis.
Fruits and Vegetables Diet Improves Kidney Functions and Electrolyte Status in Non-Insulin Dependent Diabetes Mellitus (N.I.D.D.M) Subjects
(IISTE, 2013) Olukanni, Olumide
Kidney failure and electrolyte imbalance are some of the complications associated with diabetes mellitus, if prompt treatment is not instituted it may lead to renal disease and dietary influence has been established on the prognosis of many diseases. Thus effects of fruits and vegetables diet were investigated on kidney functions and electrolyte status of some selected Non-Insulin dependent diabetic subjects at the cardiovascular clinic of Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria. The patients with mean fasting blood glucose (FBG) 7.84± 0.95 mmol dl-1 were placed on 300 g of fruits and vegetables per day in addition to their normal drugs and food intake for eight weeks. Standard laboratory methods were employed for biochemical assay; while the data were analyzed using analytic and descriptive statistics. A significant difference (p < 0.05) was noticed between baseline and eighth week’s values in the following parameters: plasma creatinine, 3.48± 0.095 to 1.79±0.57 mgdL-1; urea, 39.05±2.04-23.20±1.50 and uric acid 5.37± 0.27-2.50±0.59 mgdL-1. No significant difference (p > 0.05) was observed in plasma electrolyte values when the baseline value were compared with the eighth week’s values. After the eight weeks, the fruits and vegetables rations were stopped for two weeks and the parameters were measured again. Significant difference(p<0.05) were observed in all the values by comparing week eight with week ten values; plasma creatinine, 1.79±0.52 to 3.01± 0.68 mgdl-1,plasma urea, 39.05± 2.40 to 23.20± 1.50 mgdl-1 and plasma uric acid 2.50±0.59 to 3.61±0.50 mgd-1, however no significant difference (p> 0.05) was observed in serum electrolyte. Our results showed that fruits and vegetables consumption improved kidney function and did not affect plasma electrolytes adversely.
Genetic Variations among Three Major Ethnic Groups in Nigeria Using RAPD
(Shirazu University, Iran Molecular Biology Research Communications, 2018-10) Olukanni, Olumide
Genetically, every individual is unique; this may stem from inheritance, geographical locations, and/or environmental interactions. This study examined the possibility of developing a cheap and easy-to-use marker that can distinguish among the three ethnic groups in Nigeria using RAPD-PCR. Five RAPD primers, OPA1-3 and OPC1-2, were randomly selected and used to amplify DNA samples isolated from blood of eighteen human subjects representing the three major ethnic groups in Nigeria (six subjects each). Genomic DNAs were extracted using DNA isolation kit, RAPD-PCR amplification was performed and gel electrophoresis was done. Genetic similarity between the band polymorphism was evaluated as frequencies of occurrence and the phylogenetic tree constructed. Three of the five primers show various polymorphisms; the highest frequency band for primer OPA1 is 50% while that of primer OPA2 is 100% and for OPC2 is 83.33%. Although OPA2 has common bands in majority of the samples few of the bands are ethnic group specific. Bands 471 and 435 bp are specific for the Hausa ethnic group at 66.67% frequency. Similarly, in primer OPC2, band 320 can be used to distinguish the Hausas from the other two ethnic groups. Analysis of variance (ANOVA) and test for homogeneity showed that there is no significant difference in the polymorphism between and among the groups. In conclusion this research has given an insight into the possibility of developing RAPD primers that could be used to distinguish people of different ethnic groups.
Green Synthesis and Antibacterial Activities of Coconut Husk Extract-mediated Silver Nanoparticles
(Babcock University actaSATECH, 2018-11) Olukanni, Olumide
This work reports the synthesis of silver nanoparticles (AgNPs) using coconut husk extract and the evaluation of its antibacterial activity. Antibacterial activity of the biosynthesized AgNPs was evaluated against some selected pathogenic microorganisms. The biosynthesised AgNPs were characterised using UV-Visible, Fourier Transmission Infrared Spectroscope (FTIR), Energy Dispersive X-ray Spectroscope (EDX) and Field Emission Scanning Electron Microscope (FESEM). The absorption spectrum of the synthesized AgNPs showed a maximum spectrum of 448 nm while FTIR analysis showed different functional groups present on the surface of the AgNPs with broad peak between 839 and 3454 cm−1. The FESEM showed a large number of spherically shaped nanoparticles with sizes ranging from 12.96 to 97.04 nm. The coconut husk extract possess biomolecules which aided the bioreduction, formation and stabilization of the AgNPs, which in turn inhibited distinctly the growth of the selected microorganisms with zones ranging from 9-13 mm. This study showed that coconut husk can be used for the synthesis of AgNPs possessing high antibacterial properties.
Kinetics of the Decolorization of a Dye House Effluent by Providencia rettgeri ODO.
(International Journal of Environmental Sciences, 2013) Olukanni, Olumide
The potential of an organism isolated from a mixture of azo dyes to decolourize textile wastewater was investigated. The organism identified by 16S rDNA as a strain of Providencia rettgeri was able to remove 94 ± 0.56% of the colour in a dyehouse effluent within 24 h in batch experiments. P. rettgeri strain ODO was able to use the effluent as sole carbon and nitrogen source. The optimum pH and temperature for colour removal ranged between 7–8 and 30–35 °C, respectively. Analysis of the data showed that the decolourization process exhibited first-order kinetics with respect to the colour intensity and biomass concentration. The rate constant k=5.6 10 3 L/gDCMh at 37 °C. The specific growth rate, μ, of the organism was 0.013 h 1 while the mean doubling time was 52.9 h. The results indicated that P. rettgeri strain ODO has the potential for colour removal from textile effluent.

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